Cysteine Protease Profiles of the Medicinal Plant Calotropis procera R. Br. revealed by de novo transcriptome analysis

PloS One
Chang Woo KwonPahn-Shick Chang

Abstract

Calotropis procera R. Br., a traditional medicinal plant in India, is a promising source of commercial proteases, because the cysteine proteases from the plant exhibit high thermo-stability, broad pH optima, and plasma-clotting activity. Though several proteases such as Procerain, Procerain B, CpCp-1, CpCp-2, and CpCp-3 have been isolated and characterized, the information of their transcripts is limited to cDNAs encoding their mature peptides. Due to this limitation, in this study, to determine the cDNA sequences encoding full open reading frame of these cysteine proteases, transcripts were sequenced with an Illumina Hiseq2000 sequencer. A total of 171,253,393 clean reads were assembled into 106,093 contigs with an average length of 1,614 bp and an N50 of 2,703 bp, and 70,797 contigs with an average length of 1,565 bp and N50 of 2,082 bp using Trinity and Velvet-Oases software, respectively. Among these contigs, we found 20 unigenes related to papain-like cysteine proteases by BLASTX analysis against a non-redundant NCBI protein database. Our expression analysis revealed that the cysteine protease contains an N-terminal pro-peptide domain (inhibitor region), which is necessary for correct folding and proteolytic activity. It w...Continue Reading

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Citations

Jan 13, 2017·Applied Biochemistry and Biotechnology·Vijay L KumarMárcio Viana Ramos
Mar 22, 2018·Journal of Enzyme Inhibition and Medicinal Chemistry·Chang Woo KwonPahn-Shick Chang
May 18, 2020·Journal of Molecular Modeling·Wei Cheng PangAzzmer Azzar Abdul Hamid
Jun 4, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Márcio V RamosGerly Anne C Brito
Dec 12, 2020·Applications in Plant Sciences·Shannon C K StraubTatyana Livshultz
Jul 30, 2017·International Journal of Biological Macromolecules·Cleverson D T FreitasMárcio V Ramos

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Datasets Mentioned

BETA
SRP043253
GBZK00000000
AGI59309.1
ABQ10202.1
AAB02650

Methods Mentioned

BETA
RNA-Seq
PCR
electrophoresis
X-ray

Software Mentioned

PYMOL
InterProScan
DeconSeq
tBLASTn
Oases
Bowtie2
Modeller
BLAST
CASAVA
PROCHECK

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