Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ.

Scientific Reports
Prem KhanalXiaolong Yang

Abstract

Hippo signalling pathway is an emerging signalling pathway that plays important roles in organ size control, tumorigenesis, metastasis, stress response, apoptosis, stem cell differentiation and renewal during development and tissue homeostasis. Recent studies reported that human serine/threonine protein kinase, Mst1, a core component of the Hippo pathway can be activated through formation of homodimer. However, it is still unclear whether or not other components of the Hippo pathway are also regulated through dimerization. Here we provide the first evidence that Hippo components and oncoprotein YAP2L and TAZ can form homodimer in vitro and in vivo by forming disulphide bond through cysteine residue(s). We have also shown that the homodimers of YAP2L/TAZ are more stable and showed more oncogenic behaviour than their corresponding monomers as revealed by colony formation and cell transformation assay. Since cysteine post-translational regulation plays important roles in redox signalling, tumorigenesis and drug resistance, further studies on the functional effect of this dimerization through post-translational modulation of cysteine residues in YAP2L/TAZ will provide a significant contribution to our understanding of the roles of ...Continue Reading

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Citations

Nov 25, 2018·Nature Communications·Michael KoflerAndrás Kapus
Oct 28, 2019·Antioxidants·María José López-GruesoC Alicia Padilla
Feb 4, 2021·Physical Chemistry Chemical Physics : PCCP·Joshua L WortBela E Bode
Feb 15, 2021·Cellular and Molecular Life Sciences : CMLS·Lucy LeBlancJonghwan Kim
Sep 14, 2021·Journal of Bacteriology·Hemendra Pal Singh DhakedIndranil Biswas

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Methods Mentioned

BETA
protein folding
co-immunoprecipitation
pull down
Co-IP
immunoprecipitation
pull-down
transfection
Assay
ubiquitination

Software Mentioned

Quantity One

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