Cysteine-scanning mutagenesis study of the sixth transmembrane segment of the Na,K-ATPase alpha subunit

FEBS Letters
Saida Guennoun, Jean Daniel Horisberger

Abstract

The accessibility of the residues of the sixth transmembrane segment (TM) of the Bufo marinus Na,K-ATPase alpha subunit was explored by cysteine scanning mutagenesis. Methanethiosulfonate reagents reached only the two most extracellular positions (T803, D804) in the native conformation of the Na,K-pump. Palytoxin induced a conductance in all mutants, including D811C, T814C and D815C which showed no active electrogenic transport. After palytoxin treatment, four additional positions (V805, L808, D811 and M816) became accessible to the sulfhydryl reagent. We conclude that one side of the sixth TM helix forms a wall of the palytoxin-induced channel pore and, probably, of the cation pathway from the extracellular side to one of their binding sites.

References

Nov 1, 1989·The American Journal of Physiology·K GeeringB C Rossier
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jun 16, 1997·FEBS Letters·X Wang, J D Horisberger
Mar 7, 1998·Journal of Molecular Evolution·K B Axelsen, M G Palmgren
Jun 9, 2001·The Journal of Biological Chemistry·S AsanoN Takeguchi

❮ Previous
Next ❯

Citations

Jun 25, 2003·The Journal of Membrane Biology·M T TostesonD C Tosteson
Feb 4, 2006·The Journal of Membrane Biology·J A Hernández, J Fischbarg
Jul 20, 2007·The Journal of Membrane Biology·Saida Guennoun-LehmannRobert F Rakowski
Oct 14, 2008·Nature·Ayako TakeuchiDavid C Gadsby
Apr 27, 2007·Nature Structural & Molecular Biology·Claudia ColinaMiguel Holmgren
Mar 17, 2004·The Journal of General Physiology·Pablo Artigas, David C Gadsby
Mar 1, 2006·The Journal of General Physiology·Oihana CapendeguyJean-Daniel Horisberger
Jun 28, 2006·The Journal of General Physiology·Nadine Harmel, Hans-Jürgen Apell
Dec 19, 2002·Neuroreport·Xiaoqin YeJerold Chun
Nov 7, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David C GadsbyNicolás Reyes
Jan 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Pablo Artigas, David C Gadsby
Mar 10, 2009·Toxicon : Official Journal of the International Society on Toxinology·Chau H Wu
Mar 19, 2005·The Journal of Physiology·Oihana Capendeguy, Jean-Daniel Horisberger
May 5, 2004·The Journal of Biological Chemistry·Jean-Daniel HorisbergerOlivier Michielin
Nov 17, 2004·Advances in Physiology Education·George R Dubyak
May 24, 2003·Annals of the New York Academy of Sciences·Pablo Artigas, David C Gadsby
May 24, 2003·Annals of the New York Academy of Sciences·Jean-Daniel HorisbergerKathi Geering
Dec 28, 2002·Annals of the New York Academy of Sciences·Pablo Artigas, David C Gadsby
Aug 5, 2008·Archives of Biochemistry and Biophysics·Ramón CocaFrancisco Fernández-Belda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Haruo Ogawa, Chikashi Toyoshima
Proceedings of the National Academy of Sciences of the United States of America
Pablo Artigas, David C Gadsby
© 2022 Meta ULC. All rights reserved