PMID: 8606785Nov 1, 1995Paper

Cysteine string protein, a DnaJ family member, is present on diverse secretory vesicles

Neuropharmacology
J E Braun, R H Scheller

Abstract

Synaptic vesicles are the secretory organelles responsible for the regulated secretion of neurotransmission. Proteins associated with or integral components of the lipid bilayer likely represent important components in regulated secretion. CSP (cysteine string protein) is a 34 kDa protein which copurifies with cholinergic synaptic vesicles from the marine ray Torpedo californica. In Drosophila melanogaster deletion of the CSP gene causes impaired presynaptic neuromuscular transmission. A rat brain complementary DNA (cDNA) encoding CSP was isolated and sequence analysis predicts a protein with 86% identity to Torpedo CSP. Rat CSP contains a "J domain" as well as a cysteine rich "string" region. The J domain "fingerprints" the CSP family as members of the universally conserved DnaJ/hsp40 (heat shock protein) chaperone family. Polyclonal antisera raised against a seventeen amino acid peptide representing the carboxy terminus of rat CSP detected a 35 kDa immunoreactive protein in a rat brain synaptic vesicle enriched preparation. A 35 kDa immunoreactive protein that comigrates electrophoretically with rat brain CSP was also detected in zymogen granule membranes. These results establish the presence of a CSP in rat brain and in the ...Continue Reading

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Citations

Jun 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·H ChinC A Kozak
Jul 3, 2008·Cell Stress & Chaperones·Alma Rosales-HernandezJanice E A Braun
Aug 15, 2013·Archives of Biochemistry and Biophysics·Hiromi ShimomuraTomoko Nashida
Feb 1, 2003·Biochemical and Biophysical Research Communications·Leigh Anne SwayneJanice E A Braun
May 1, 1997·Trends in Neurosciences·E Buchner, C B Gundersen
Jun 23, 2007·Journal of Proteome Research·Michael J RindlerThomas A Neubert
Aug 7, 1998·Molecular Biology of the Cell·L H Chamberlain, R D Burgoyne
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·F BenfenatiS Giovedí
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jadah N JohnsonJanice E A Braun
Nov 15, 2000·Canadian Journal of Physiology and Pharmacology·H Fisher, J E Braun
Apr 1, 1999·Annual Review of Physiology·R Fernández-Chacón, T C Südhof
Mar 14, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ning WengGuy E Groblewski
Mar 4, 2008·American Journal of Respiratory Cell and Molecular Biology·Joungjoa ParkKenneth B Adler
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·L De VriesM G Farquhar
Sep 16, 2005·Molecular and Cellular Neurosciences·Leigh Anne SwayneJanice E A Braun
Oct 28, 2008·Biochemical and Biophysical Research Communications·Gerald R PrescottAlan Morgan
Nov 7, 2008·Biology of the Cell·Anton EvdoninNatalia Medvedeva
Aug 1, 2006·Biochemical and Biophysical Research Communications·Leigh Anne SwayneJanice E A Braun
Sep 29, 2004·Biochemical and Biophysical Research Communications·Sanja SelakMarvin J Fritzler
Nov 23, 2006·Biochemical and Biophysical Research Communications·Liping BaiJanice E A Braun
Jun 30, 2010·Journal of Neurogenetics·Konrad E Zinsmaier
Mar 25, 2015·Seminars in Cell & Developmental Biology·Robert D Burgoyne, Alan Morgan
Aug 12, 1996·FEBS Letters·T Coppola, C Gundersen
Oct 15, 1998·FEBS Letters·A MastrogiacomoC B Gundersen
May 9, 2014·Frontiers in Cellular Neuroscience·Julien Donnelier, Janice E A Braun
Jul 30, 2014·Frontiers in Cellular Neuroscience·Carolina Koutras, Janice E A Braun

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