Cystic fibrosis transmembrane conductance regulator mediates tenogenic differentiation of tendon-derived stem cells and tendon repair: accelerating tendon injury healing by intervening in its downstream signaling

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Yang LiuGang Li

Abstract

Tendons are a mechanosensitive tissue, which enables them to transmit to bone forces that are derived from muscle. Patients with tendon injuries, such as tendinopathy or tendon rupture, were often observed with matrix degeneration, and the healing of tendon injuries remains a challenge as a result of the limited understanding of tendon biology. Our study demonstrates that the stretch-mediated activation channel, cystic fibrosis transmembrane conductance regulator (CFTR), was up-regulated in tendon-derived stem cells (TDSCs) during tenogenic differentiation under mechanical stretching. Tendon tissues in CFTR-dysfunctional DF508 mice exhibited irregular cell arrangement, uneven fibril diameter distribution, weak mechanical properties, and less matrix formation in a tendon defect model. Moreover, both tendon tissues and TDSCs isolated from DF508 mice showed significantly decreased levels of tendon markers, such asscleraxis,tenomodulin,Col1A1(collagen type I α 1 chain), anddecorinFurthermore, by RNA sequencing analysis, we demonstrated that Wnt/β-catenin signaling was abnormally activated in TDSCs from DF508 mice, thereby further activating the pERK1/2 signaling pathway. Of most importance, we found that intervention in pERK1/2 sig...Continue Reading

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Citations

Sep 30, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tao WangMing H Zheng
Mar 30, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tao WangMing H Zheng
Mar 1, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shanshan XieHongbin Lu
Mar 30, 2019·Stem Cell Research & Therapy·Yang LiuGang Li
Apr 18, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Weiping LinGang Li
Jan 28, 2020·Journal of Cellular Physiology·Valérie OrlandoGeneviève Mailhot
Feb 6, 2020·Frontiers in Genetics·Pauline Po Yee Lui, Chi Ming Wong
Mar 20, 2020·Cell Death & Disease·Minhao ChenYunfeng Rui
Oct 10, 2020·World Journal of Stem Cells·Hao-Nan WangGuo-Xin Ni
Mar 3, 2021·Stem Cell Reviews and Reports·Bing Wei, Jun Lu
Mar 9, 2021·Frontiers in Cell and Developmental Biology·Zizhan HuangWeiliang Shen
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Yuwei YangHongwei Ouyang
Oct 3, 2021·Npj Regenerative Medicine·Paula Sarmiento, Dianne Little
Jan 4, 2022·Advances in Wound Care·Xue XuZhong Zheng

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