Cyt toxin expression reveals an inverse regulation of insect and plant virulence factors of Dickeya dadantii

Environmental Microbiology
Denis CostechareyreG Condemine

Abstract

The plant pathogenic bacteria Dickeya dadantii is also a pathogen of the pea aphid Acyrthosiphon pisum. The genome of the bacteria contains four cyt genes, encoding homologues of Bacillus thuringiensis Cyt toxins, which are involved in its pathogenicity to insects. We show here that these genes are transcribed as an operon, and we determined the conditions necessary for their expression. Their expression is induced at high temperature and at an osmolarity equivalent to that found in the plant phloem sap. The regulators of cyt genes have also been identified: their expression is repressed by H-NS and VfmE and activated by PecS. These genes are already known to regulate plant virulence factors, but in an opposite way. When tested in a virulence assay by ingestion, the pecS mutant was almost non-pathogenic while hns and vfmE mutants behaved in the same way as the wild-type strain. Mutants of other regulators of plant virulence, GacA, OmpR and PhoP, that do not control Cyt toxin production, also showed reduced pathogenicity. In an assay by injection of bacteria, the gacA strain was less pathogenic but, surprisingly, the pecS mutant was slightly more virulent. These results show that Cyt toxins are not the only virulence factors req...Continue Reading

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Citations

Dec 30, 2011·Molecular Plant-microbe Interactions : MPMI·Isabel Rio-AlvarezEmilia López-Solanilla
Jun 19, 2012·Annual Review of Phytopathology·Amy CharkowskiIris Yedidia
Dec 17, 2014·Toxins·Leopoldo PalmaPrimitivo Caballero
Oct 12, 2013·Environmental Microbiology Reports·Sylvie Reverchon, William Nasser
Jun 8, 2012·Molecular Plant Pathology·John MansfieldGary D Foster
Jan 22, 2011·FEMS Microbiology Reviews·Geetanchaly Nadarasah, John Stavrinides
Mar 6, 2015·Scientific Reports·Karine LothPedro da Silva

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