Cytidine and uridine increase striatal CDP-choline levels without decreasing acetylcholine synthesis or release.

Cellular and Molecular Neurobiology
Ismail H UlusR J Wurtman

Abstract

Treatments that increase acetylcholine release from brain slices decrease the synthesis of phosphatidylcholine by, and its levels in, the slices. We examined whether adding cytidine or uridine to the slice medium, which increases the utilization of choline to form phospholipids, also decreases acetylcholine levels and release. We incubated rat brain slices with or without cytidine or uridine (both 25-400 microM), and with or without choline (20-40 microM), and measured the spontaneous and potassium-evoked release of acetylcholine. Striatal slices stimulated for 2 h released 2650+/-365 pmol of acetylcholine per mg protein when incubated without choline, or 4600+/-450 pmol/mg protein acetylcholine when incubated with choline (20 microM). Adding cytidine or uridine (both 25-400 microM) to the media failed to affect acetylcholine release whether or not choline was also added, even though the pyrimidines (400 microM) did enhance choline;s utilization to form CDP-choline by 89 or 61%, respectively. The pyrimidines also had no effect on acetylcholine release from hippocampal and cortical slices. Cytidine or uridine also failed to affect acetylcholine levels in striatal slices, nor choline transport into striatal synaptosomes. These da...Continue Reading

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Citations

Jun 4, 2008·Clinical and Experimental Pharmacology & Physiology·Naciye Isbil-BuyukcoskunIsmail H Ulus
Apr 30, 2009·Annual Review of Nutrition·Richard J WurtmanIsmail H Ulus
Apr 1, 2008·European Journal of Pharmacology·Eline M van der Beek, Patrick J G H Kamphuis
Jul 22, 2008·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Mehmet CansevIsmail H Ulus
Sep 28, 2015·Restorative Neurology and Neuroscience·Turkan KoyuncuogluTulin Alkan
Dec 30, 2014·Neuropharmacology·Mehmet CansevRichard J Wurtman
May 16, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Esra Orenlili YaylagulSerap Celikler Kasimogullari
Jun 13, 2013·Nutrition·Weiqian MiPatrick J G H Kamphuis
Aug 9, 2017·Oxidative Medicine and Cellular Longevity·Dhaval Patel, Stephan N Witt
Jun 27, 2019·Progress in Retinal and Eye Research·Muneeb A FaiqKevin C Chan

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