Cytochalasin J affects chromosome congression and spindle microtubule organization in PtK1 cells

Cell Motility and the Cytoskeleton
J A Snyder, L Cohen

Abstract

PtK1 cells were treated with 10 micrograms/ml cytochalasin J (CJ) for 15 min at various stages of mitosis. When applied at nuclear envelope breakdown (NEB) chromosome congression was blocked or substantially slowed, and chromosomes failed to show organization patterns typical of prometaphase. Spindle microtubule (MT) numbers appeared unaffected as judged by the pattern of birefringent retardation. However, ultrastructural analysis showed MTs to be reorganized within the spindle domain with some exhibiting fragmentation and others failing to interact with poorly defined kinetochore laminae. The spindle domain took on a curved, almost banana-like shape, as related to the position of the centrosomes and lack of orientation of chromosomes. Serial section analysis of kinetochore regions showed reduced contour length and maturation of the kinetochore plate with few MTs associated with this structure. Cells similarly treated with 10 micrograms/ml CJ at NEB for 15 min and then released into conditioned medium for 15 min showed the most chromosomes resumed congression to the metaphase plate. Ultrastructural analysis revealed a more normal organization of spindle MTs, but kinetochore structure remained affected. CJ treatment of cells in ...Continue Reading

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Citations

Jul 25, 1998·Cell Motility and the Cytoskeleton·X WuJ A Hammer
Jan 25, 2002·Cell Motility and the Cytoskeleton·R V Silverman-Gavrila, A Forer
Oct 21, 1999·Cell Biology International·J LamprechtN Paweletz
Aug 3, 2001·Cell Biology International·G A Wrench, J A Snyder
Apr 28, 2005·Planta·Hiroshi YasudaShin-Ichiro Ejiri
Feb 11, 2012·Quarterly Reviews of Biophysics·J Richard McIntoshFazly I Ataullakhanov
Mar 23, 2010·Cellular and Molecular Life Sciences : CMLS·Helder Maiato, Mariana Lince-Faria
May 13, 2003·Cell Motility and the Cytoskeleton·Rosalind V Silverman-Gavrila, Arthur Forer
Sep 17, 2011·Developmental Cell·Joshua C SandquistWilliam M Bement
Aug 26, 2017·Science·Helder Maiato, Cristina Ferrás

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