PMID: 8609366Feb 1, 1996Paper

Cytochemical localization of ecto-ATPases in rat neurohypophysis

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
S ThirionG Nicaise

Abstract

We studied the distribution of Ca(2+)- or Mg(2+)-dependent ATPase activity in rat neurohypophysis using the lead cytochemical method of Ando et al. In electron microscopy, precipitates were found lining the outer surface of the plasma membrane surrounding nerve endings and pituicytes. These precipitates were believed to represent the activity of ecto-ATPases (as opposed to Ca pump ATPases) for the following reasons: there was equal activation by Ca2+ in the absence of Mg2+ or Mg2+ in the absence of Ca2+; the effects of the two ions were not additive; there was activation by ATP or GTP; and there was resistance to glutaraldehyde fixation, to high (10 mM) Ca2+ concentrations, and to various inhibitors such as NEM, vanadate, oligomycin, quercetin, p-chloromercuribenzoate, ouabain, and levamisole. Cytosolic activity observed in certain nerve endings in the same conditions of incubation but more sensitive to NEM is also described and discussed.

Citations

Dec 21, 2002·The European Journal of Neuroscience·Lia RossoJean-Marc Mienville
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
May 10, 2001·Cell Structure and Function·A BouchotJ M Pinon
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Oct 6, 2009·Trends in Endocrinology and Metabolism : TEM·Stanko S Stojilkovic
Mar 30, 2007·Biology of the Cell·Patricia M PiersonLia Rosso
Sep 23, 2008·Glia·Lia Rosso, Jean-Marc Mienville
May 16, 2008·Journal of Cellular Physiology·Thomas K KnottJosé R Lemos
Feb 22, 2012·Cell Calcium·José R LemosGovindan Dayanithi
May 12, 2015·Autonomic Neuroscience : Basic & Clinical·Ivana BjelobabaStanko S Stojilkovic
Mar 8, 2018·Journal of Neuroendocrinology·José R LemosSonia Ortiz-Miranda
Nov 11, 2006·Journal of Cellular Physiology·Thomas K KnottJosé R Lemos
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Nov 6, 2009·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·P S ManoonkitiwongsaR L Schultz

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