Cytochemical observation of regulated bacterial beta-galactosidase gene expression in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America
H S LiuP J Stambrook

Abstract

Bacterial beta-galactosidase, encoded by the lacZ gene, serves as a sensitive cytochemical marker in eukaryotic cells and tissues. In transient expression experiments, human and simian cells stain blue 48 hr after transfection with a plasmid containing a lacZ gene, whose expression is directed by a simian virus 40 promoter containing a synthetic lactose operator sequence. Transfection efficiency was about 0.6%. Incorporation of an operator sequence within the promoter permits regulation of beta-galactosidase gene expression by the lacI gene product, the lac repressor. When cells were cotransfected with the lacZ plasmid and a second plasmid containing the lacI gene, beta-galactosidase activity was extinguished. Its activity could be reestablished to original levels upon application of isopropyl beta-D-thiogalactoside to transfected cells. A cell line that stably carries both the lacI and lacZ genes was efficiently induced to synthesize beta-galactosidase after isopropyl beta-D-thiogalactoside administration. In transient expression experiments and in stably transfected lines, repression and induction of beta-galactosidase activity were predominantly at the transcriptional level.

References

Aug 24, 1978·Nature·P J Farabaugh
Sep 12, 1988·Nucleic Acids Research·M K DushP J Stambrook
Aug 22, 1986·Science·M C Mossing, M T Record
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko
Jan 1, 1986·Annual Review of Cell Biology·C Dingwall, R A Laskey
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G N Pavlakis, D H Hamer
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J Majors, H E Varmus

❮ Previous
Next ❯

Citations

May 1, 1994·Transgenic Research·C CuiP J Stambrook
Feb 28, 1992·Biochimica Et Biophysica Acta·D S BiardA Sarasin
Nov 1, 1996·Nucleic Acids Research·P R KramerR R Sinden
Jun 19, 2001·Genes & Development·C A CroninH Scrable
Jul 1, 1991·Baillière's Clinical Haematology·A Hagenbeek, A C Martens
Jul 6, 2021·Frontiers in Genetics·Sierra S NishizakiAlan P Boyle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.