PMID: 6537958Mar 1, 1984Paper

Cytochemical studies of adenylate cyclase in the choroid plexus and brain vessels of rat and mouse

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
A W VorbrodtD H Dobrogowska

Abstract

Adenylate cyclase (AC) activity was studied in the choroid plexus and brain vessels of rat and mouse using a recently improved cytochemical method (Palkama A, Kaufman HE, Uusitalo R, Uusitalo H, Virtanen J, Huhtaniitty S: J Histochem Cytochem 29:898, 1981). For comparison, the vasculature of skeletal muscle was also examined. Specimens were fixed for 5 min in 1% paraformaldehyde or 1% paraformaldehyde and 0.1% glutaraldehyde supplemented with activators of AC: isoproterenol and 5'-guanylylimidodiphosphate or forskolin. These activators were also present in the "activating solution" and in the incubation medium. For control purposes no activators were used. Various capturing agents were introduced into incubation media: SrCl2, Pb citrate, NdCl3, and CeCl3. Satisfactory results were obtained with both fixatives, both activators, and the capturing agents SrCl2 and Pb citrate. In rat choroid plexus AC activity was localized in the basal and lateral plasmalemma of epithelial cells, in the plasmalemma of cells interposed between epithelium and capillaries and occasionally in pinocytotic vesicles of endothelial cells. In micro-blood vessels of rat brain, the reaction appeared in the luminal or in both luminal and abluminal plasmalemma...Continue Reading

Citations

Apr 29, 1998·Microscopy Research and Technique·P A Richards, P D Richards
Oct 12, 2001·Microscopy Research and Technique·J P SleemanP Baumann
Jan 1, 1985·Histochemistry·L Rechardt, H Hervonen
May 1, 1988·The Histochemical Journal·G Poeggel, H Luppa
Jan 1, 1986·Neuroscience and Biobehavioral Reviews·G C Palmer
Jun 7, 2003·Brain Research. Brain Research Reviews·Andrzej W Vorbrodt, Danuta H Dobrogowska
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·K J HalbhuberN Zimmermann
Jan 1, 1988·Progress in Histochemistry and Cytochemistry·A W Vorbrodt
Jul 1, 1988·Stroke; a Journal of Cerebral Circulation·W I Rosenblum

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