Cytochemical studies on peroxisomes in rat and guinea pig myocardium

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
G Asano, M Ashraf

Abstract

The enzymatic activity and distribution of peroxisomes (microbodies) in rat and guinea pig hearts were studied cytochemically, by means of oxidation of 3-3'-diaminobenzidine (DAB) and by using B-glycerophosphate and cytidine-5'-monophosphate as substrates. Peroxisomes were localized in proximity to mitochondria and sarcoplasmic reticulum and measured from 0.2 micrometers to 0.5 micrometers in diameter in both animal species. DAB positive bodies were seen both at pH 9.0 and pH 5.0 in rat myocardial cells. However, in guinea pig myocardial cells the reaction was observed only at pH 9.0, or very faintly at pH 5.0. Acid and alkaline phosphatases were not demonstrated in the peroxisomes. Lipid droplets were surrounded by a ring of dense granular reaction product for enzymes, such as acid and alkaline phosphatase, and lipofuscin granules were limited by acid phosphatase or DAB reaction products. The pathophysiological function of peroxisomes is discussed.

References

Apr 1, 1976·Journal of Molecular and Cellular Cardiology·V Herzog, H D Fahimi
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A B Novikoff
Jan 1, 1977·Journal of Molecular and Cellular Cardiology·T W WangJ J Lech
May 1, 1972·The Journal of Cell Biology·P M Novikoff, A B Novikoff
Sep 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M BorgersW Schaper
Jan 1, 1972·Annual Review of Biophysics and Bioengineering·G Inesi
Nov 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C De Duve
Nov 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E McGroarty, N E Tolbert
Nov 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J K Reddy
Nov 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A B NovikoffC Davis
Aug 1, 1969·The Journal of Cell Biology·M E Beard, A B Novikoff
Mar 1, 1980·Journal of Molecular and Cellular Cardiology·G AsanoA Schwartz

❮ Previous
Next ❯

Citations

Jan 1, 1992·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·S Yokota, K Asayama
Oct 28, 2003·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Goro AsanoYuhichi Sugisaki
Apr 23, 2013·Clinical & Experimental Ophthalmology·Hassan I H El-SayyadEzaldin A M Mohammed

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.