Cytochemistry and immunolocalisation of polysaccharides and proteoglycans in the endosperm of green Arabica coffee beans

Protoplasma
Paul W SutherlandR J Redgwell

Abstract

The major noncellulosic polysaccharides and proteoglycans in the coffee bean (Coffea arabica) cell wall are (galacto)mannans and arabinogalactan proteins. Immunological and chemical probes demonstrated that the mannans and arabinogalactan proteins were located continuously across the width of the cell wall, but that the concentration of different structural epitopes within these polysaccharide types showed considerable spatial variation. For the mannans this was implied by the striated pattern demonstrated by fluctuation of the affinity between the mannan monoclonal antibody BGM C6 and (galacto)mannan. The arabinogalactan proteins labelled by the Yariv reagent and the arabinogalactan protein-specific antibody LM2 appeared to be located in all regions of the wall except the middle lamella, but showed some differences in intensity of labelling. However, the LM6 antibody, specific for (1-->5)-alpha-arabinan epitopes, was located only as a compact region adjacent to the cell lumen in the body of the endosperm; though, it did label throughout the wall of epidermal cells. This implied that either some of the more highly arabinosylated arabinogalactan proteins contained contiguous 5-arabinosyl residues or that a rhamnogalacturonan whi...Continue Reading

Citations

Nov 13, 2010·Journal of Experimental Botany·Magdalena OpanowiczSinéad Drea
Nov 10, 2013·Journal of Experimental Botany·Thierry JoëtStéphane Dussert
Aug 21, 2014·Annals of Botany·Derek T A LamportCharlotte E Seal
Aug 19, 2014·Carbohydrate Polymers·Ana S P MoreiraManuel A Coimbra
Apr 26, 2007·Journal of Agricultural and Food Chemistry·Fernando M Nunes, Manuel A Coimbra
Aug 17, 2006·Journal of Agricultural and Food Chemistry·Naoya KasaiGou Maeda

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