PMID: 4291334Dec 1, 1966Paper

Cytochemistry of phosphatases of the sarcoplasmic reticulum. I. Biochemical studies

The Journal of Cell Biology
A G Engel, L W Tice

Abstract

A microsomal fraction was isolated from rabbit psoas muscle by a modification of Muscatello's method. The fraction contained a Mg-dependent ATPase which had a pH optimum of 7.5. Activity was further stimulated by addition of Na or K or other monovalent cations to the reaction mixture, but synergistic activation by Na and K, and ouabain inhibition, could not be demonstrated. The enzyme hydrolyzed only ATP (adenosine triphosphate) and ITP (inosine triphosphate) at appreciable rates, but Na or K stimulated activity only when ATP was used as substrate. Activity was inhibited by Ca and by low concentrations of Na deoxycholate, and was sensitive to inhibition by thiol group reagents. The enzyme could be distinguished from another enzyme, also present in the fraction, which was Ca-activated, and which exhibited a wider substrate specificity, different pH activation characteristics, lower specific activity, lack of stimulation by Na or K, and less sensitivity to inhibition by deoxycholate and by thiol group reagents. These findings formed the basis for demonstration of the Mg-dependent ATPase in situ.

References

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Citations

Jan 1, 1971·Histochemie. Histochemistry. Histochimie·B Agostini, W Hasselbach
Jan 1, 1967·Histochemie. Histochemistry. Histochimie·G F Gauthier
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·R A Sabbadini, A S Dahms
Oct 7, 1977·Molecular and Cellular Biochemistry·D R HeadonJ O'Flaherty
Feb 1, 1971·Irish Journal of Medical Science·P F Duggan
Jan 1, 1983·The International Journal of Biochemistry·J T Fitzsimons
Sep 1, 1967·The Journal of Cell Biology·J R Sommer, W Hasselbach
Nov 1, 1972·The Journal of Cell Biology·J A Heuson-StiennonP Drochmans
Jan 1, 1968·J R Microsc Soc·A M Glauert
Jun 7, 2014·Journal of Cellular Physiology·Elin Hadler-OlsenLars Uhlin-Hansen
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·K J HalbhuberN Zimmermann
Sep 1, 1979·Experimental Cell Research·N N Malouf, G Meissner
Sep 1, 1968·The American Journal of Cardiology·T N James, L Sherf
Jan 1, 1974·International Journal of Health Services : Planning, Administration, Evaluation·P D Stolley

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