PMID: 1654817Jul 1, 1991Paper

Cytochrome c-catalyzed oxidation of organic molecules by hydrogen peroxide

Archives of Biochemistry and Biophysics
R RadiE Prodanov


Cytochrome c catalyzed the oxidation of various electron donors in the presence of hydrogen peroxide (H2O2), including 2-2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 4-aminoantipyrine (4-AP), and luminol. With ferrocytochrome c, oxidation reactions were preceded by a lag phase corresponding to the H2O2-mediated oxidation of cytochrome c to the ferric state; no lag phase was observed with ferricytochrome c. However, brief preincubation of ferricytochrome c with H2O2 increased its catalytic activity prior to progressive inactivation and degradation. Superoxide (O2-) and hydroxyl radical (.OH) were not involved in this catalytic activity, since it was not sensitive to superoxide dismutase (SOD) or mannitol. Free iron released from the heme did not play a role in the oxidative reactions as concluded from the lack of effect of diethylenetriaminepentaacetic acid. Uric acid and tryptophan inhibited the oxidation of ABTS, stimulation of luminol chemiluminescence, and inactivation of cytochrome c. Our results are consistent with an initial activation of cytochrome c by H2O2 to a catalytically more active species in which a high oxidation state of an oxo-heme complex mediates the oxidative reactions. The lack of SOD effect...Continue Reading


Jan 1, 1990·Journal of Bioluminescence and Chemiluminescence·G MerényiT E Eriksen
Jan 1, 1990·Free Radical Biology & Medicine·Rafael RadiE Prodanov
Jan 1, 1989·Free Radical Biology & Medicine·H C Sutton, C C Winterbourn
Feb 1, 1985·Journal of Inorganic Biochemistry·T M Florence
Jan 1, 1986·Annual Review of Physiology·I Fridovich, B A Freeman
Jan 1, 1985·Journal of Free Radicals in Biology & Medicine·S D AustC E Thomas
Jan 1, 1987·Free Radical Biology & Medicine·H B Dunford
Jul 1, 1972·The Biochemical Journal·A BoverisB Chance
Dec 1, 1973·Photochemistry and Photobiology·E K Hodgson, I Fridovich
Apr 15, 1982·Archives of Biochemistry and Biophysics·H P Misra, P M Squatrito
Jan 14, 1982·Brain Research·T D SteegeA R Wyler


Dec 19, 2001·Microscopy Research and Technique·C L Murrant, M B Reid
Sep 15, 1994·Biochimica Et Biophysica Acta·M Pérez-GilabertF García-Carmona
Jun 15, 1994·Biochemical Pharmacology·M Pérez-GilabertF García-Carmona
Feb 1, 1994·Free Radical Biology & Medicine·N W KooyJ S Beckman
Mar 17, 2004·Journal of Pharmaceutical and Biomedical Analysis·Suiping ZhouAmandio Vieira
Feb 22, 2001·Free Radical Biology & Medicine·Rafael RadiA Cayota
Jul 24, 1998·The International Journal of Biochemistry & Cell Biology·M A RoseiC Cini
Nov 19, 2011·Biochemistry·Gordon J Hildick-SmithRoger K Sandwick
Aug 19, 2007·Chemical Communications : Chem Comm·Joshua R PetersonPall Thordarson
Aug 8, 2001·European Journal of Biochemistry·R E DiederixG W Canters
Feb 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Luiz C GodoyJoan B Mannick
Oct 3, 2006·Biochemistry. Biokhimii︠a︡·Yu A VladimirovValerian E Kagan
May 1, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Wenjun Zhang, Genxi Li
Apr 26, 2013·Cellular and Molecular Life Sciences : CMLS·Sobia ZaidiFaizan Ahmad
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Mar 17, 2009·Free Radical Biology & Medicine·Valerian E KaganGrigory Borisenko
Apr 2, 2010·BMB Reports·Jung Hwan YoonJung Hoon Kang
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Jan 25, 2007·Journal of Biochemistry and Molecular Biology·Nam Hoon Kim, Jung Hoon Kang
Jan 25, 2007·Journal of Biochemistry and Molecular Biology·Hiroaki NagaiHidenori Ichijo
May 29, 2012·Biochemical and Biophysical Research Communications·Huiyong YinL Jackson Roberts
Apr 26, 2000·Chemistry & Biology·J A VillegasR Vazquez-Duhalt
Jan 20, 2015·Molecular and Cellular Endocrinology·Paola VendittiSergio Di Meo
Dec 9, 2014·Biochimica Et Biophysica Acta·Alexander M FirsovYuri N Antonenko
Jul 1, 1991·Archives of Biochemistry and Biophysics·Rafael RadiB A Freeman
Apr 4, 2014·Chemical Reviews·Luisa B Maia, José J G Moura
Dec 30, 2017·Chembiochem : a European Journal of Chemical Biology·Małgorzata SierantBarbara Nawrot
May 28, 1998·The Journal of Biological Chemistry·L J DeterdingK B Tomer
Mar 18, 2006·The Journal of Biological Chemistry·Irina I VlasovaValerian E Kagan
Oct 15, 2010·Free Radical Research·L CastroRafael Radi
Apr 20, 2000·The Journal of Biological Chemistry·A M CassinaRafael Radi
Jan 1, 1993·Toxicology and Industrial Health·R Radi
Mar 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Blas Moreno-BeltránIrene Díaz-Moreno
Oct 18, 1996·The Journal of Biological Chemistry·M C KrishnaA Samuni
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Jan 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Stefan HaywardPieter Swart
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