PMID: 8606159Apr 1, 1996Paper

Cytochrome cb-type nitric oxide reductase with cytochrome c oxidase activity from Paracoccus denitrificans ATCC 35512

Journal of Bacteriology
T Fujiwara, Y Fukumori

Abstract

A highly active nitric oxide reductase was purified from Paracoccus denitrificans ATCC 35512, formerly named Thiosphaera pantotropha, which was anaerobically cultivated in the presence of nitrate. The enzyme was composed of two subunits with molecular masses of 34 and 15 kDa and contained two hemes b and one heme c per molecule. Copper was not found in the enzyme. The spectral properties suggested that one of the two hemes b and heme c were in six-coordinated low-spin states and another heme b was in a five-coordinated high-spin state and reacted with carbon monoxide. The enzyme showed high cytochrome c-nitric oxide oxidoreductase activity and formed nitrous oxide from nitric oxide with the expected stoichiometry when P. denitrificans ATCC 35512 ferrocytochrome c-550 was used as the electron donor. The V max and Km values for nitric oxide were 84 micromol of nitric oxide per min/mg of protein and 0.25 microM, respectively. Furthermore, the enzyme showed ferrocytochrome c-550-O2 oxidoreductase activity with a V max of 8.4 micromol of O2 per min/mg of protein and a Km value of 0.9 mM. Both activities were 50% inhibited by about 0.3 mM KCN.

References

Jul 15, 1990·The Biochemical Journal·G J Carr, S J Ferguson
Jan 15, 1994·European Journal of Biochemistry·B SamynJ J van Beeumen
Feb 15, 1994·European Journal of Biochemistry·B C BerksS J Ferguson
Mar 15, 1994·Biochemistry·J A García-HorsmanR B Gennis
Mar 14, 1994·FEBS Letters·M Saraste, J Castresana
Jan 1, 1993·Archives of Microbiology·W G Zumft
Jan 1, 1988·Annual Review of Microbiology·L I Hochstein, G A Tomlinson
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

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Citations

Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·A GiuffrèT Soulimane
Jul 9, 2002·The Journal of Biological Chemistry·Cláudio M GomesMiguel Teixeira
Jan 19, 2006·Biochemical Society Transactions·U FlockP Adelroth
Jun 17, 2008·Biotechnology and Bioengineering·Peter van der MaasPiet Lens
Jan 24, 2012·Nature Structural & Molecular Biology·Yushi MatsumotoYoshitsugu Shiro
Feb 5, 2008·The Journal of Biological Chemistry·Jessica H van WonderenJulea N Butt
Mar 18, 2010·Chembiochem : a European Journal of Chemical Biology·Valentin Köhler, Thomas R Ward
Sep 10, 2013·The Journal of Biological Chemistry·Josy Ter BeekPia Ädelroth
Sep 28, 2014·FEBS Letters·Vivek Sharma, Mårten Wikström
Dec 15, 1996·European Journal of Biochemistry·A P de BoerR J van Spanning
Jul 3, 1998·FEBS Letters·M S MuntyanN P Starshinova
Dec 7, 2007·The Journal of Biological Chemistry·Ulrika FlockPia Adelroth
Feb 24, 2015·PloS One·Seda AratReinhard Laubenbacher
Oct 27, 2017·Frontiers in Microbiology·Margarida M SantanaCristina Cruz
Jan 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroki YoneharaEtsuro Yoshimura
Jun 19, 2004·Journal of Bacteriology·Hubertus J E BeaumontRob J M van Spanning
Jul 15, 2017·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Yafei SunHexin Fei
Apr 4, 2021·International Journal of Environmental Research and Public Health·Wen Jia, Liuyan Yang
Sep 27, 2000·Biochimica Et Biophysica Acta·J HendriksM Saraste
Apr 11, 2001·Journal of Inorganic Biochemistry·S KuroseT Sakurai
May 13, 1999·Biochimica Et Biophysica Acta·N J WatmoughS Spiro
Mar 5, 2003·Free Radical Biology & Medicine·Paolo SartiMaurizio Brunori
Nov 14, 1997·Journal of Bacteriology·R CrammB Friedrich
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·W G Zumft

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