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Cytochrome P-450 measurement in rat liver homogenate and microsomes. Its use for correction of microsomal losses incurred by differential centrifugation

Drug Metabolism and Disposition : the Biological Fate of Chemicals

Jan 1, 1975

Jean Paul JolyY Peasant

PMID: 1235

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Abstract

Cytochrome P-450 was assayed in rat liver homogenates and microsomes in order to calculate microsomal recoveries and correct for losses during ultracentrifugation or sedimentation in presence of CaCl2. The values obtained for corrected microsomal protein in untreated female Sprague-Dawl...read more

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Cytochrome P450
Centrifugation
NADH
Flavoproteins
Luminal
Cytochrome P-450 Oxygenase
Ultracentrifugation
Succinate Dehydrogenase
Sedimentation Procedure
Assay
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  • Cytochrome P-450 measurement in rat liver homogenate and microsomes. Its use for correction of microsomal losses incurred by differential centrifugation

    Drug Metabolism and Disposition : the Biological Fate of Chemicals

    Jan 1, 1975

    Jean Paul JolyY Peasant

    PMID: 1235

    DOI:

    Abstract

    Cytochrome P-450 was assayed in rat liver homogenates and microsomes in order to calculate microsomal recoveries and correct for losses during ultracentrifugation or sedimentation in presence of CaCl2. The values obtained for corrected microsomal protein in untreated female Sprague-Dawl...read more

    Mentioned in this Paper

    Cytochrome P450
    Centrifugation
    NADH
    Flavoproteins
    Luminal

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