PMID: 7297566Sep 1, 1981Paper

Cytochrome P-450 monooxygenase activities in human and rat liver microsomes

European Journal of Biochemistry
P KremersJ E Gielen


Microsomes were prepared from human livers obtained from renal donors of various ages and both sexes. Their drug-metabolizing capacity was measured and compared to that of rat liver microsomes. The following parameters were investigated: cytochrome P-450, cytochrome B5, NADPH-cytochrome c reductase, epoxide hydrolase, aryl hydrocarbon hydroxylase, benzphetamine N-demethylase, p-nitroanisole-O-demethylase, ethoxycoumarin-O-deethylase, steroid-16 alpha-hydroxylase. In addition, the metabolism of benzo(a)pyrene, progesterone, pregnenolone, testosterone, dehydroepiandrosterone and estradiol was studied in detail in vitro. The inhibitory effect of metyrapone and alpha-naphthoflavone on 7-ethoxycoumarin-O-deethylase was measured. The microsomal proteins of both species were separated by polyacrylamide gel electrophoresis in the presence of dodecyl sulfate. The following conclusions were drawn from the results obtained. Human liver microsomes can be stored under optimal conditions for the measurement of a large variety of enzymic activities. Human liver microsomes are able to metabolize the various xenobiotics used as substrates with a rate similar to that of female rat liver microsomes. No sex-linked difference in enzymic activity wa...Continue Reading


Jan 20, 1975·Biochemical and Biophysical Research Communications·R FleischmannH Remmer
Jan 1, 1979·Drug Metabolism Reviews·A P AlvaresA H Conney
Jan 1, 1979·Pharmacology & Therapeutics·F P Guengerich
Jun 13, 1979·Biochemical and Biophysical Research Communications·P BeauneJ P Leroux
Oct 1, 1979·Clinical Pharmacology and Therapeutics·G C FarrellL W Powell
Dec 30, 1977·Archives of Toxicology·V Ullrich, P Kremers
Nov 21, 1977·Biochemical and Biophysical Research Communications·J Van CantfortJ E Gielen
Jan 1, 1978·Methods in Enzymology·R W Estabrook, J Werringloer
Feb 1, 1977·Clinical Pharmacology and Therapeutics·J KapitulnikA H Conney
Mar 1, 1976·Journal of Steroid Biochemistry·R J BegueP Padieu
Jan 2, 1975·Biochemical and Biophysical Research Communications·V UllrichP Wollenberg
Sep 1, 1973·Clinical Pharmacology and Therapeutics·O PelkonenN T Kärki
Jul 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·V Ullrich, P Weber
Apr 1, 1980·European Journal of Clinical Pharmacology·E A SotaniemiM Puukka
Jan 1, 1980·Archives of Biochemistry and Biophysics·P WangF P Guengerich
Jan 1, 1980·British Journal of Clinical Pharmacology·A R BoobisD S Davies
Jun 1, 1980·Clinical Pharmacology and Therapeutics·C von BahrH Glaumann
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J FishmanA Kappas


Jul 1, 1994·Journal of Cellular Physiology·A BuardP Laurent
Jan 1, 1994·Cancer Chemotherapy and Pharmacology·L MassaadA Gouyette
Jan 1, 1992·European Journal of Drug Metabolism and Pharmacokinetics·W D HooperP E Reilly
Nov 13, 2010·European Journal of Nuclear Medicine and Molecular Imaging·Steffen FischerPeter Brust
Sep 15, 1994·Biochemical Pharmacology·F BottoY Barra
Jan 1, 1988·Pharmacology & Therapeutics·P Beaune, F P Guengerich
Oct 22, 2002·Biochemical and Biophysical Research Communications·Jean-Philippe BarqueJacqueline Bonaly
Sep 1, 1992·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·T Wolff, M Strecker
Nov 1, 1987·Baillière's Clinical Endocrinology and Metabolism·L Agius
Jun 1, 1990·British Journal of Clinical Pharmacology·D SesardicD S Davies
Dec 1, 1989·European Journal of Clinical Investigation·N PerrotP Beaune
Dec 27, 1990·European Journal of Biochemistry·J P RenaudD Mansuy
Sep 18, 2004·European Journal of Biochemistry·Joel A KrauserF P Guengerich
Mar 23, 2011·The Journal of Pharmacology and Experimental Therapeutics·Gang XieBasil Rigas
Aug 5, 2000·Biochemical and Biophysical Research Communications·A S Moubarak, C F Rosenkrans
Jan 1, 1990·Journal of Enzyme Inhibition·J O JohnstonH D Benson
Sep 1, 1988·The American Journal of the Medical Sciences·S M MitrioneN G Abraham
Dec 22, 1999·Chemico-biological Interactions·J M NicolasJ Roba

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