Cytochrome P-450 revealed: the effect of the respiratory cytochromes on the spectrum of bacterial cytochrome P-450

Biochemical and Biophysical Research Communications
P M StevensonA J Fulco

Abstract

Soluble extracts of Bacillus megaterium ATCC 14581 prepared by centrifuging a sonicated cell suspension at 40,000 xg for 30 min apparently contained no cytochrome P-450 unless the culture had been grown in the presence of an inducer: a reduced+CO minus reduced spectrum was used to measure cytochrome P-450 concentration. When the 40,000 xg supernatants from the uninduced cultures were recentrifuged at 105,000 xg the respiratory cytochromes, including one like cytochrome a1, were sedimented, and cytochrome P-450 was observed to be 100 nM or 30 +/- 9 p mol cytochrome P-450/mg protein (n=9). Measurements of cytochrome P-450 in cultures induced with phenobarbital were always higher after ultracentrifugation. There was soluble cytochrome o in all extracts. When cytochrome a1 was present a deep trough at 441 nm developed in the reduced +CO minus reduced difference spectrum of the 40,000 xg supernatant of both the uninduced and the induced cultures. The 40,000 xg supernatant obtained after lysing protoplasts of B. megaterium did not contain cytochrome a1 and always gave a good measure of cytochrome P-450.

References

Dec 29, 1978·Biochemical and Biophysical Research Communications·J F Buchanan, A J Fulco
May 1, 1975·CRC Critical Reviews in Microbiology·P JurtshukW C Acord
Sep 6, 1967·Biochimica Et Biophysica Acta·C W Jones, E R Redfearn
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Jan 1, 1970·Annual Review of Biochemistry·M D Kamen, T Horio
Dec 16, 1980·Biochemical and Biophysical Research Communications·R S MatsonA J Fulco

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Citations

Jan 1, 1990·Archives of Microbiology·J B SutherlandC E Cerniglia
Jan 1, 1990·Biomaterials·R WasserbauerB Dolezel
Oct 16, 1984·Biochimica Et Biophysica Acta·R T RuettingerA J Fulco
Jul 1, 1986·Applied and Environmental Microbiology·J B Sutherland
Nov 15, 1983·Biochemical and Biophysical Research Communications·L O NarhiA J Fulco

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