Cytochrome P450 expression profile of the PICM-19H pig liver cell line: potential application to rapid liver toxicity assays.

In Vitro Cellular & Developmental Biology. Animal
Ryan R WillardT J Caperna

Abstract

Liver in vitro models are needed to replace animal models for rapid assessment of drug biotransformation and toxicity. The PICM-19 pig liver stem cell line may fulfill this need since these cells have activities associated with xenobiotic phase I and II metabolism lacking in other liver cell lines. The objective of this study was to characterize phase I and II metabolic functions of a PICM-19 derivative cell line, PICM-19H, compared to the tumor-derived human HepG2 C3A cell line and primary cultures of adult porcine hepatocytes. Following exposure of PICM-19H cells to either 3-methylcholanthrene, rifampicin or phenobarbital, the induced activities of cytochrome P450 (CYP450) isozymes CYP-1A, -2, and-3A were assessed. Relative to adult porcine hepatocytes, PICM- 19H cells exhibited 30% and 43%, respectively, of CYP1A and 3A activities, while HepG2 C3A cells exhibited 7% and 0% of those activities. Fluorescent metabolites were extensively conjugated, i.e., 52% and 96% of CYP450-1A and-3A metabolites were released from medium samples following treatment with β-glucuronidase/arylsulfatase. Rifampicin induction of CYP450 isozyme activities was confirmed by conversion of testosterone to 6β-OH-, 2α-OH- and 2β-OH-testosterone, as deter...Continue Reading

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Citations

Jul 8, 2010·In Vitro Cellular & Developmental Biology. Animal·Neil C TalbotThomas J Caperna
Feb 8, 2011·In Vitro Cellular & Developmental Biology. Animal·Thomas J CapernaNeil C Talbot
Nov 24, 2012·The Journal of General Virology·Sophie RogéeNicole Pavio
Aug 24, 2020·Biotechnology Progress·Carlota OleagaJames J Hickman
Mar 17, 2020·Archives of Toxicology·Martin Krøyer Rasmussen

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