Cytochrome P450 metabolites of arachidonic acid: rapid incorporation and hydration of 14,15-epoxyeicosatrienoic acid in arterial smooth muscle cells

Prostaglandins, Leukotrienes, and Essential Fatty Acids
X FangArthur A Spector

Abstract

Arachidonic acid is converted to epoxyeicosatrienoic acids (EETs) by cytochrome P450 monooxygenases. EETs produce arterial vasodilatation, and recent evidence suggests that they are endothelium-derived hyperpolarizing factors. In porcine coronary arteries contracted with a thromboxane mimetic agent, we find that relaxation is rapidly initiated by exposure to 14,15-EET. The relaxation slowly increases in magnitude, resulting in a response which is sustained for more than 10 min. Cultured porcine aortic smooth muscle cells rapidly take up [3H]14,15-EET. After 3 min, radioactivity is present in neutral lipids, phosphatidylcholine, and phosphatidylinositol. The cells also convert 14,15-EET to 14,15-dihydroxyeicosatrienoic acid (14,15-DHET), and some DHET is detected in the medium after only 1 min of incubation. Like 14,15-EET, 14,15-DHET produces relaxation of the contracted coronary artery rings. These findings suggest that the incorporation into phospholipids and conversion to 14,15-DHET can occur at a rate that is fast enough to modulate the vasorelaxation produced by 14,15-EET.

References

Dec 1, 1995·Circulation·R A Cohen, P M Vanhoutte
Jan 1, 1995·British Journal of Pharmacology·D FultonJ Quilley
Jan 1, 1995·Trends in Pharmacological Sciences·C J GarlandT M Cocks
Apr 1, 1994·Kidney International·R A GirtonJ A Gordon

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Citations

Oct 21, 2010·Journal of Agricultural and Food Chemistry·Karen WagnerBruce D Hammock
Aug 19, 2009·Antioxidants & Redox Signaling·Valery N BochkovJohannes Stöckl
Feb 5, 2011·Clinical and Experimental Pharmacology & Physiology·Wei PangYi Zhu
Nov 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Brandon T LarsenDavid D Gutterman
Feb 3, 2012·Physiological Reviews·John D Imig
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Benjamin B DavisRobert H Weiss
Apr 26, 2006·European Journal of Clinical Investigation·B T LarsenO A Hatoum
May 4, 2010·Cardiovascular Therapeutics·Hong QiuNipavan Chiamvimonvat
Jan 29, 2011·Congestive Heart Failure·Ning LiNipavan Chiamvimonvat
Apr 23, 2013·Drug Metabolism Reviews·Abdulaziz M S AlsaadAyman O S El-Kadi
May 12, 2009·Chemistry and Physics of Lipids·Pei H CuiMichael Murray
Jul 31, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Xiang FangArthur A Spector
Oct 27, 2001·Circulation Research·I Fleming
Aug 23, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Xiang FangArthur A Spector
Sep 22, 2006·American Journal of Physiology. Cell Physiology·Arthur A Spector, Andrew W Norris
Sep 23, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Mohammed A NayeemS Jamal Mustafa
Jun 28, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·I Fleming, R Busse
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·K G MaierR J Roman
Dec 11, 2003·American Journal of Physiology. Renal Physiology·Zhigang YuRobert H Weiss
Jul 29, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Asma YaghiDavid G McCormack
Dec 9, 1998·The American Journal of Physiology·X FangA A Spector
Apr 25, 2000·Archives of Biochemistry and Biophysics·J F GreeneB D Hammock
Mar 8, 2005·Progress in Lipid Research·John W NewmanBruce D Hammock
Dec 14, 2006·Prostaglandins & Other Lipid Mediators·Houli Jiang

❮ Previous
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