PMID: 7536247Jan 15, 1995Paper

Cytochrome P450 mono-oxygenase-regulated signalling of Ca2+ entry in human and bovine endothelial cells

The Journal of Physiology
W F GraierM Sturek

Abstract

1. We tested the hypothesis that agonist-stimulated Ca2+ entry, and thus formation of endothelium-derived nitric oxide (EDNO) in vascular endothelial cells, is related to activation of microsomal P450 mono-oxygenase (P450 MO) and the biosynthesis of 5,6-epoxyeicosatrienoic acid (5,6-EET). 2. Several P450 inhibitors diminished the sustained [Ca2+]i plateau response to agonist or intracellular Ca2+ store depletion with ATPase inhibitors by 31-69% (fura-2 technique). Mn2+ influx stimulated by agonists or ATPase inhibitors was prevented by P450 inhibitors. 3. Histamine- or ATPase inhibitor-stimulated formation of EDNO was strongly attenuated (50-83%) by P450 inhibitors, without any effect on EDNO formation by the Ca2+ ionophore A23187, indicating that decreased EDNO synthesis is due specifically to the inhibition of Ca2+ entry by these compounds. 4. Induction of P450 MO by beta-naphthoflavone potentiated agonist-induced Ca2+ and Mn2+ influx by 60 and 53%, respectively. Intracellular Ca2+ release remained unchanged. 5. The P450 MO product, 5,6-EET (< 156 nmol l-1), activated Ca2+/Mn2+ entry without any depletion of intracellular Ca2+ stores. The 5,6-EET-stimulated Ca2+/Mn2+ entry was not affected by P450 inhibitors. 6. As with the b...Continue Reading

Citations

Oct 6, 2001·Biochemical and Biophysical Research Communications·D K BowlesM Sturek
Jun 12, 2013·Pflügers Archiv : European journal of physiology·David C EllinsworthShaun L Sandow
Mar 22, 2014·In Vitro Cellular & Developmental Biology. Animal·Yukari UedaEiichi Sakurai
Apr 10, 2010·Pflügers Archiv : European journal of physiology·Cor de Wit, Tudor M Griffith
Jun 9, 1998·European Journal of Pharmacology·B G Hoebel, W F Graier
Dec 27, 1995·European Journal of Pharmacology·W F GraierG M Kostner
Apr 12, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Robert Morfin
Mar 31, 2000·Trends in Pharmacological Sciences·J Quilley, J C McGiff
Nov 1, 1996·Progress in Cardiovascular Diseases·P M Vanhoutte, J V Mombouli
May 14, 1999·British Journal of Pharmacology·J V MombouliW F Graier
Jul 30, 2005·American Journal of Respiratory and Critical Care Medicine·Diego F AlvarezMary I Townsley
Jan 12, 2002·British Journal of Pharmacology·Victor E EsenabhaluWolfgang F Graier
Nov 28, 2013·Expert Opinion on Drug Metabolism & Toxicology·Andrew Rowland, Arduino A Mangoni
Sep 16, 2005·Endothelium : Journal of Endothelial Cell Research·Nour B BisharaMichael A Hill
Sep 17, 2009·Immunological Reviews·James W Putney
Aug 26, 2010·British Journal of Pharmacology·Alexander BondarenkoWolfgang F Graier
Jan 16, 1998·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J BauersachsR Busse
Feb 17, 1998·Biochemical Pharmacology·J QuilleyJ C McGiff
May 18, 2005·Microvascular Research·Natalia V BogatchevaAlexander D Verin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.