Cytochrome P450 monooxygenases and insecticide resistance in insects

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
Jean-Baptiste BergéMarcel Amichot

Abstract

Cytochrome P450 monooxygenases are involved in many cases of resistance of insects to insecticides. Resistance has long been associated with an increase in monooxygenase activities and with an increase in cytochrome P450 content. However, this increase does not always account for all of the resistance. In Drosophila melanogaster, we have shown that the overproduction of cytochrome P450 can be lost by the fly without a corresponding complete loss of resistance. These results prompted the sequencing of a cytochrome P450 candidate for resistance in resistant and susceptible flies. Several mutations leading to amino-acid substitutions have been detected in the P450 gene CYP6A2 of a resistant strain. The location of these mutations in a model of the 3D structure of the CYP6A2 protein suggested that some of them may be important for enzyme activity of this molecule. This has been verified by heterologous expression of wild-type and mutated cDNA in Escherichia coli. When other resistance mechanisms are considered, relatively few genetic mutations are involved in insecticide resistance, and this has led to an optimistic view of the management of resistance. Our observations compel us to survey in more detail the genetic diversity of cy...Continue Reading

References

Apr 29, 2009·Ecotoxicology·John M Brausch, Philip N Smith
Nov 26, 2013·Pesticide Biochemistry and Physiology·L MaBing Li
Feb 11, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·M E Hahn
Jun 27, 2003·Insect Biochemistry and Molecular Biology·G Le GoffR H ffrench-Constant
Sep 17, 2002·Insect Molecular Biology·Hilary RansonF H Collins
Mar 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Henry ChungPhillip J Daborn
Mar 20, 2004·European Journal of Biochemistry·Marcel AmichotJean-Baptiste Bergé
Apr 13, 2000·Annual Review of Entomology·Janet Hemingway, Hilary Ranson
Dec 9, 2000·Annual Review of Entomology·T G Wilson
Mar 9, 2012·Parasites & Vectors·Mo'awia Mukhtar HassanHind Mohammed Abushama
Feb 5, 2008·Journal of the American Mosquito Control Association·María M RodríguezDitter Fernández
Jun 8, 2011·Future Medicinal Chemistry·Debra J WoodsPatrick F Meeus
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Michael R BogwitzPhillip J Daborn
Jan 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Pavan KumarIan T Baldwin
Jul 21, 2000·Journal of Economic Entomology·Phillip J DabornP Batterham
Nov 5, 2013·Journal of Medical Entomology·Leslie C AlvarezAdriana E Flores
Sep 8, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G Vázquez-GómezM E Heres-Pulido
Nov 11, 2008·European Journal of Cell Biology·Umesh GangishettiBernard Moussian
Jul 10, 2004·Insect Biochemistry and Molecular Biology·Janet HemingwayHilary Ranson
Nov 14, 2006·Insect Biochemistry and Molecular Biology·Lee WilloughbyPhillip J Daborn

Citations

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·L C WatersL Y Ch'ang
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M R Frank, J C Fogleman
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L C WatersL Y Ch'ang
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R FeyereisenD W Nebert
Dec 1, 1988·Genome Génome / Conseil National De Recherches Canada·D R HouptR A Morton
Mar 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M J CoonL L Bestervelt
Jan 1, 1996·Archives of Insect Biochemistry and Physiology·J G ScottN Liu
Jul 1, 1996·Molecular Biology and Evolution·M Taylor, R Feyereisen
Jan 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M Zhang, J G Scott
Jun 1, 1997·Insect Biochemistry and Molecular Biology·B R PittendrighR H ffrench-Constant
Jun 1, 1997·Insect Biochemistry and Molecular Biology·R L RoseE Hodgson
Feb 17, 1999·Annual Review of Entomology·R Feyereisen

Related Concepts

Genes, Insect
Cyp6a2 protein, Drosophila
Cyp6a2
Alkalescens-Dispar Group
Drosophila melanogaster Proteins
Cytochrome P450
Insecticide Resistance Function
Cytochrome P-450 Oxygenase
Gene Expression
Sequencing

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.