Cytochrome P450 side-chain cleavage enzyme in the cerebellar Purkinje neuron and its neonatal change in rats

Endocrinology
K UkenaK Tsutsui

Abstract

Neurosteroids are de novo synthesized in the nervous system through mechanisms at least partly independent of peripheral steroidogenic glands. However, the concept of neurosteroidogenesis in neurons is not clear in mammalian brains. The present study identified the presence of cytochrome P450scc in the rat Purkinje cell, a typical cerebellar neuron. Immunohistochemical analysis with the antibody against the purified bovine adrenal P450scc showed an immunoreaction restricted to somata and dendrites of the Purkinje cells in adult cerebella. Preadsorbing the antibody with P450scc resulted in a complete absence of the immunoreaction. The antibody against inositol triphosphate receptor, a marker of the Purkinje cell, recognized P450scc-immunoreactive cerebellar cells that showed no immunoreaction with glial fibrillary acidic protein, a specific marker of glial cells. Expression of the P450scc-like protein in the cerebellum was verified by Western blot analysis, and cerebellar P450scc messenger RNA, by RT-PCR analysis in adulthood. On the other hand, P450scc-immunoreactive cells were found to scatter throughout the cerebellum at 0 day of age, before the differentiation of the first Purkinje cells, while the site of expression of this...Continue Reading

Citations

Mar 1, 2008·Endocrinology·Paul Micevych, Kevin Sinchak
Dec 26, 2001·Trends in Endocrinology and Metabolism : TEM·Synthia H Mellon, Lisa D Griffin
Feb 6, 2004·Pharmacology & Therapeutics·Deborah A FinnJohn C Crabbe
Feb 12, 2011·General and Comparative Endocrinology·Sakae Kikuyama, Kazuyoshi Tsutsui
Jul 27, 2007·Pharmacology & Therapeutics·Synthia H Mellon
Nov 23, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Kazuyoshi Tsutsui
Feb 21, 2006·The European Journal of Neuroscience·Manuel Mameli, C Fernando Valenzuela
Jun 15, 2013·Journal of Neuroendocrinology·G BiaginiS Meletti
Apr 27, 2011·Journal of Cellular Physiology·Yen-Feng ChiangMeng-Chun Hu
Aug 22, 2006·Research in Veterinary Science·Gul Fatma YarimMurat Yarim
May 22, 2009·Annals of the New York Academy of Sciences·Jean-Luc Do RegoHubert Vaudry
Aug 22, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Lisa WesselCarsten Theiss
Aug 12, 2006·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Kazuyoshi TsutsuiKazuyoshi Ukena
Jan 12, 2007·Genesis : the Journal of Genetics and Development·Hsu-Shui WuMeng-Chun Hu
Jul 7, 2007·The Journal of Comparative Neurology·Princy S QuadrosChristine K Wagner
Jul 24, 2010·Journal of Neurochemistry·Fang FangNazzareno Ballatori
Nov 2, 2005·The Journal of Comparative Neurology·Emi KiyokageKazunori Ishimura
Sep 10, 2003·Endocrinology·Hirotaka SakamotoKazuyoshi Tsutsui
Mar 1, 2008·Endocrinology·Kazuyoshi Tsutsui

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.