Cytochrome P450s from the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae): Expression profiles of different stages and responses to host allelochemicals

Insect Biochemistry and Molecular Biology
Lulu DaiHui Chen

Abstract

Bark beetles oxidize the defensive allelochemicals from their host trees to both detoxify and convert these materials into components of their pheromone system. The ability of this insect to adapt to its chemical environment might be explained by the action of major detoxification enzymes such as cytochrome P450s (or CYPs). Sixty-four sequences coding for P450s were identified, and most of the transcripts were found to be expressed in the larvae, pupae and adults of Dendroctonus armandi. To gain information on how these genes help D. armandi overcome the host defense, differential transcript levels of the CYP genes were observed between sexes and within the sexes. Significant differences were observed among developmental stages, in feeding on the phloem of Pinus armandi and in exposure to stimuli ((±)-α-pinene, (S)-(-)-α-pinene, (S)-(-)-β-pinene, (+)-3-carene, (±)-limonene and turpentine oil) for 8 h. We investigated the effect of sex and generations on the survivorship of individual D. armandi that were exposed to host volatiles at concentrations comparable to constitutive and induced levels of defense using fumigant exposure to understand the ability of the beetles to tolerate host defensive chemicals. The differential transc...Continue Reading

References

Feb 11, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·J G ScottZ Wen
Feb 17, 1999·Annual Review of Entomology·R Feyereisen
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·James T WarrenLawrence I Gilbert
Oct 19, 2006·Insect Biochemistry and Molecular Biology·Pamela SandstromClaus Tittiger
Nov 1, 2006·Biochemical Society Transactions·R Feyereisen
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Oct 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Si Quang LeOlivier Gascuel
Nov 27, 2008·Journal of Chemical Ecology·Pamela SandstromClaus Tittiger
Aug 26, 2010·Biochimica Et Biophysica Acta·David R Nelson
Oct 5, 2010·Biochimica Et Biophysica Acta·Mary A Schuler
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Feb 5, 2013·Insect Biochemistry and Molecular Biology·Minmin SongClaus Tittiger
Mar 5, 2013·Insect Biochemistry and Molecular Biology·María Fernanda LópezGerardo Zúñiga
Mar 30, 2013·Genome Biology·Christopher I KeelingJoerg Bohlmann
Dec 2, 2014·The American Naturalist·Barbara J Bentz, James A Powell
Dec 2, 2014·The American Naturalist·Jeffry B Mitton, Scott Ferrenberg

❮ Previous
Next ❯

Citations

Jun 28, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Lulu DaiHui Chen
Jul 26, 2018·Journal of Chemical Ecology·Christine C ChiuJoerg Bohlmann
Dec 29, 2020·Current Opinion in Insect Science·Gary J BlomquistChristopher I Keeling

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.