Cytochrome rC552, formed during expression of the truncated, Thermus thermophilus cytochrome c552 gene in the cytoplasm of Escherichia coli, reacts spontaneously to form protein-bound 2-formyl-4-vinyl (Spirographis) heme

Biochemistry
James A FeeA Pastuszyn

Abstract

Expression of the truncated (lacking an N-terminal signal sequence) structural gene of Thermus thermophilus cytochrome c(552) in the cytoplasm of Escherichia coli yields both dimeric (rC(557)) and monomeric (rC(552)) cytochrome c-like proteins [Keightley, J. A., et al. (1998) J. Biol. Chem. 273, 12006-12016], which form spontaneously without the involvement of cytochrome c maturation factors. Cytochrome rC(557) is comprised of a dimer and has been structurally characterized [McRee, D., et al. (2001) J. Biol. Chem. 276, 6537-6544]. Unexpectedly, the monomeric rC(552) transforms spontaneously to a cytochrome-like chromophore having, in its reduced state, the Q(oo) transition (alpha-band) at 572 nm (therefore called p572). The X-ray crystallographic structure of rC(552), at 1.41 A resolution, shows that the 2-vinyl group of heme ring I is converted to a [heme-CO-CH(2)-S-CH(2)-C(alpha)] conjugate with cysteine 11. Electron density maps obtained from isomorphous crystals of p572 at 1.61 A resolution reveal that the 2-vinyl group has been oxidized to a formyl group. This explains the lower energy of the Q(oo)() transition, the presence of a new, high-frequency band in the resonance Raman spectra at 1666 cm(-1) for oxidized and at 164...Continue Reading

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Citations

Nov 26, 2008·Natural Product Reports·Sarah E J Bowman, Kara L Bren
Apr 6, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·F Ann Walker
Jun 23, 2012·Protein & Cell·Elizabeth B Sawyer, Paul D Barker
Apr 29, 2015·Biochimica Et Biophysica Acta·Ying-Wu Lin
Jun 25, 2005·Chembiochem : a European Journal of Chemical Biology·Ying-Wu LinHou-Ming Wu
Oct 7, 2004·The Journal of Biological Chemistry·Takeshi UchidaTeizo Kitagawa
Feb 27, 2018·Journal of Inorganic Biochemistry·Hui-Min ChengYing-Wu Lin

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