Cytogenetic Analysis of the Results of Genome Editing on the Cell Model of Parkinson's Disease

Bulletin of Experimental Biology and Medicine
A S VetchinovaS N Illarioshkin

Abstract

We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in LRRK2 gene associated with Parkinson's disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed. These data confirm the importance of the development of new approaches ensuring genome stability in CRISPR/Cas9-edited cultures.

References

Jun 10, 2008·Lancet Neurology·Daniel G HealyUNKNOWN International LRRK2 Consortium
Mar 4, 2011·Nature·Samer M HusseinTimo Otonkoski
Jan 5, 2013·Science·Le CongFeng Zhang
Mar 16, 2013·RNA Biology·Ambarish BiswasChris M Brown
Apr 25, 2014·Nature Communications·Peter S Choi, Matthew Meyerson
May 6, 2014·Biochimica Et Biophysica Acta·Tobias WijshakeBart van de Sluis
Jan 28, 2016·Scientific Reports·Xiaoxi LiuToru Takumi
Apr 8, 2017·Nature Medicine·Tatjana I CornuToni Cathomen

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Citations

Nov 28, 2019·Cellular and Molecular Neurobiology·Fatemeh SafariFarzaneh Khademi
Jul 2, 2021·Frontiers in Molecular Neuroscience·Xiaolin ZhuHubin Duan
Nov 24, 2021·Molecular Neurobiology·Lihong GuanJuntang Lin

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