Cytogenetic analysis using simultaneous and sequential fluorescence in situ hybridization

Cancer Genetics and Cytogenetics
T EscuderoJ Egozcue

Abstract

A sequential fluorescence in situ hybridization (FISH) technique is described. This method allows the detection of up to eighteen chromosome pairs in consecutive hybridizations (8 steps) on the same metaphase using centromeric, whole chromosome painting, and single copy DNA probes with different fluorochromes. The technique may be used with diagnostic purposes in cases with poor cytogenetic material.

References

Nov 1, 1992·Trends in Genetics : TIG·J S Heslop-HarrisonI J Leitch
Apr 1, 1996·Nature Genetics·M M Le Beau
Apr 1, 1996·Nature Genetics·M R SpeicherD C Ward
Jul 26, 1996·Science·E SchröckT Ried

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Citations

Jun 14, 2002·Journal of Reproductive Immunology·Josep EgozcueF Vidal
Sep 16, 2000·Molecular and Cellular Endocrinology·J EgozcueF Vidal
May 25, 2015·Neuroscience and Biobehavioral Reviews·Vladan BajicThomas Arendt
Aug 10, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·P E Stanley, L J Kricka
Jun 27, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M J FerrariP M Strebel

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