Cytogenetic and molecular characterization of intergeneric hybrids between Brassica napus and Orychophragmus violaceus

Genome Génome / Conseil National De Recherches Canada
B F ChengD J Somers

Abstract

Twenty-two intergeneric hybrids from a cross between Brassica napus (AACC, 2n = 38) cultivar Oro and the ornamental crucifer Orychophragmus violaceus (OO, 2n = 24) were produced without embryo rescue. The plants were classified into three groups based on morphological and cytological observations and RAPD banding patterns. Plants of Group I had morphological traits of both parents and 2n = 29 chromosomes. In these plants, 62.1% of the pollen mother cells (PMCs) had the pairing configuration 1 III + 9 II + 8 I; the remaining PMCs had 10 II + 9 I. The plants possessed 97.6-98.8% B. napus specific and 9.2-11.7% O. violaceus specific RAPD fragments. Plants of Group II exhibited novel morphological traits and possessed 2n = 35, 36, or 37 chromosomes. Plants of Group III were morphologically similar to B. napus and possessed 2n = 19, 37, 38, or 39 chromosomes. Plants of Group II and Group III had 94.1-99.4% B. napus specific RAPD fragments and no O. violaceus specific RAPD fragments. Chromosome fragments were observed in PMCs of most of the F1 plants in all groups. Based on the cytological results and RAPD analysis, it is suggested that genome doubling and chromosome elimination occurred in the intergeneric hybrids of B. napus x O. v...Continue Reading

References

Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·K SongT C Osborn
Aug 1, 1994·Genome Génome / Conseil National De Recherches Canada·B F ChengB Y Chen
Aug 1, 1999·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Z Li, W K Heneen
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Citations

Jul 19, 2013·Plant Cell Reports·Xian-Hong GeZai-Yun Li
Dec 14, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Michaela MarkovaJitka Zluvova
Feb 19, 2014·The Journal of Physical Chemistry. B·Quentin R JohnsonTongye Shen
Jan 15, 2014·Chemical Reviews·Sethuramasundaram PitchiayaNils G Walter
Jul 15, 2015·Biotechnology Advances·Sangam L DwivediRodomiro Ortiz
Sep 19, 2006·Environmental Biosafety Research·Henriette Ammitzbøll, Rikke Bagger Jørgensen
Feb 25, 2006·Genome Génome / Conseil National De Recherches Canada·C F MarfilL Comai
May 26, 2021·Plant Biotechnology Journal·Dandan HuJun Zou

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