Cytogenetic and Sequence Analyses of Mitochondrial DNA Insertions in Nuclear Chromosomes of Maize

G3 : Genes - Genomes - Genetics
Ashley N LoughKathleen J Newton

Abstract

The transfer of mitochondrial DNA (mtDNA) into nuclear genomes is a regularly occurring process that has been observed in many species. Few studies, however, have focused on the variation of nuclear-mtDNA sequences (NUMTs) within a species. This study examined mtDNA insertions within chromosomes of a diverse set of Zea mays ssp. mays (maize) inbred lines by the use of fluorescence in situ hybridization. A relatively large NUMT on the long arm of chromosome 9 (9L) was identified at approximately the same position in four inbred lines (B73, M825, HP301, and Oh7B). Further examination of the similarly positioned 9L NUMT in two lines, B73 and M825, indicated that the large size of these sites is due to the presence of a majority of the mitochondrial genome; however, only portions of this NUMT (~252 kb total) were found in the publically available B73 nuclear sequence for chromosome 9. Fiber-fluorescence in situ hybridization analysis estimated the size of the B73 9L NUMT to be ~1.8 Mb and revealed that the NUMT is methylated. Two regions of mtDNA (2.4 kb and 3.3 kb) within the 9L NUMT are not present in the B73 mitochondrial NB genome; however, these 2.4-kb and 3.3-kb segments are present in other Zea mitochondrial genomes, includi...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 10, 1998·Nature Genetics·P SanMiguelJ L Bennetzen
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D R Piperno, K V Flannery
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·R M StuparJ Jiang
May 23, 2001·Trends in Ecology & Evolution·D BensassonG M. Hewitt
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoshihiro MatsuokaJohn Doebley
Sep 26, 2002·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Zhukuan ChengJiming Jiang
Jan 22, 2004·Nature Reviews. Genetics·Jeremy N TimmisWilliam Martin
Mar 12, 2004·Molecular Biology and Evolution·Erik Richly, Dario Leister
Jul 16, 2004·Molecular Biology and Evolution·Erik Richly, Dario Leister
Sep 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Akio KatoJames A Birchler
Sep 14, 2004·PLoS Biology·Miria RicchettiBernard Dujon
Nov 16, 2004·Plant Physiology·Sandra W CliftonKathleen J Newton
Dec 1, 2004·Annual Review of Genetics·John Doebley
Aug 12, 2005·International Journal of Biological Sciences·Minoru UedaKoh-ichi Kadowaki
Dec 20, 2005·The Plant Journal : for Cell and Molecular Biology·Sherry A Flint-GarciaEdward S Buckler
Feb 7, 2006·Current Opinion in Plant Biology·Edward S BucklerMichael D McMullen
Aug 21, 2007·Annals of Botany·Masanori YamasakiMichael D McMullen
Jan 19, 2008·Genetics·Jianming YuEdward S Buckler
Aug 5, 2008·FEMS Yeast Research·Christine SacerdotOdile Ozier-Kalogeropoulos
Nov 19, 2008·Annual Review of Plant Biology·Tatjana KleineDario Leister
Jan 3, 2009·PLoS Genetics·Anna E Sheppard, Jeremy N Timmis
Dec 8, 2009·Science·Patrick S SchnableRichard K Wilson
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Feb 20, 2010·PLoS Genetics·Einat Hazkani-CovoWilliam Martin
Mar 4, 2010·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Dal-Hoe KooYoonkang Hur
Jul 16, 2010·Database : the Journal of Biological Databases and Curation·Taner Z SenCarolyn J Lawrence
Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mihaela Maria MartisAndreas Houben
May 15, 2013·Nucleic Acids Research·Hamish McWilliamRodrigo Lopez

❮ Previous
Next ❯

Citations

Mar 11, 2019·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jiming Jiang
May 13, 2020·Genome Génome / Conseil National De Recherches Canada·María J Puertas, Mónica González-Sánchez
Jan 3, 2018·PloS One·Jéssica Coutinho SilvaWellington Ronildo Clarindo
Oct 25, 2020·The Plant Journal : for Cell and Molecular Biology·Shin-Ichi ArimuraNobuhiro Tsutsumi

❮ Previous
Next ❯

Software Mentioned

Adobe Photoshop
Align Sequences Nucleotide BLAST
ZeAlign
RepeatMasker
InterProScan
BLAST
MaizeGDB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.