Abstract
Bladder cancer is frequently associated with chromosomal abnormalities, and the complexity of karyotypes increases with tumor progression. The murine model MB49 is one of the most widely studied models of bladder cancer. We developed the invasive cell line MB49-I by successive in vivo passages of MB49 primary tumors. Because little is known about the chromosomal alterations of this model, our goal was to perform cytogenetic analyses of the MB49 and MB49-I lines. The karyotypes of both lines were analyzed by G-banding and fluorescence in situ hybridization techniques. Both lines were composed of two cell subpopulations, a diploid population, which was found mainly in the MB49 line, and the tetraploid population, which was found mainly in the MB49-I line. A translocation between chromosomes 5 and 9 and an isochromosome of chromosome 19 were observed in the subpopulations of both lines. New structural abnormalities and additional chromosomal imbalances were detected in the MB49-I line. Tumor progression in the MB49/MB49-I model was associated with a selection of polyploid cells with accompanying chromosomal abnormalities. This model may be advantageous for the study of the genetic changes associated with the progression of bladder...Continue Reading
References
Feb 19, 2000·Genes, Chromosomes & Cancer·J J GildeaD Theodorescu
Mar 16, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Hong-Lin ChengNan-Haw Chow
Oct 31, 2002·American Journal of Medical Genetics·Avery A Sandberg
Jul 13, 2004·European Urology·Antonio Lopez-Beltran, Rodolfo Montironi
May 3, 2005·International Journal of Surgical Pathology·Rodolfo Montironi, Antonio Lopez-Beltran
May 11, 2005·Urology·José PlacerAntoni Gelabert-Mas
Jul 16, 2005·Urologic Oncology·Seth P Lerner
Mar 4, 2006·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Nov 15, 2007·Cytogenetic and Genome Research·D J Wolff
Dec 5, 2008·Scandinavian Journal of Urology and Nephrology. Supplementum·Carlos Cordon-Cardo
Dec 24, 2008·Proteomics·Manousos MakridakisAntonia Vlahou
Feb 21, 2009·International Journal of Cancer. Journal International Du Cancer·Spasenija SavicLukas Bubendorf
May 19, 2009·BMC Cancer·Karsten ZiegerLars Dyrskjøt
Jun 23, 2009·The Journal of Urology·Catalina LodillinskyAna María Eiján
Oct 27, 2009·The Journal of Urology·Fanghong ChenWilliam A See
Feb 12, 2010·Future Oncology·Luca Toschi, Federico Cappuzzo
Apr 7, 2010·Cancer Genetics and Cytogenetics·Min-Jung SongSun-Hee Kim
May 5, 2010·Pathology·Sarah MinnerWaldemar Wilczak
May 13, 2010·Genes, Chromosomes & Cancer·Sarah V WilliamsMargaret A Knowles
Oct 5, 2010·Japanese Journal of Clinical Oncology·Yoshiyuki KakehiAkira Yokomizo
Mar 31, 2011·Science Signaling·Annette S LittleSimon J Cook
Apr 12, 2011·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Patrick L WagnerSven Perner
Citations
Apr 19, 2015·European Journal of Medicinal Chemistry·Jovane Hamelin-MorrissetteGervais Bérubé
Nov 12, 2013·Nitric Oxide : Biology and Chemistry·Denise BelgoroskyAna María Eiján
Jan 25, 2018·Journal of Cellular Biochemistry·Barbara Prack Mc CormickAna M Eiján
Jan 26, 2020·Journal of Biomolecular Structure & Dynamics·P ChanphaiH A Tajmir-Riahi
May 8, 2018·Bladder Cancer·Tahlita C M ZuiverloonDan Theodorescu
Mar 15, 2019·Oncology Letters·Marina AlbertóValeria Inés Segatori
Mar 27, 2018·Journal of Cellular Physiology·Denise BelgoroskyBetiana Lerner
Oct 4, 2018·Histochemistry and Cell Biology·Andreja ErmanPeter Veranič
Mar 13, 2021·Frontiers in Pharmacology·Changkuo ZhouHu Guo
Mar 18, 2021·Clinical Oncology : a Journal of the Royal College of Radiologists·A WilkinsN Sundahl
Jul 3, 2021·International Journal of Molecular Sciences·Andreja ErmanRok Romih