Cytogenetic damage after ischemia and reperfusion

Genetic Testing and Molecular Biomarkers
Theodore LialiarisKonstantinos Natsis

Abstract

Tourniquets are often used to provide a bloodless operating field. However, they carry the risk of adverse effects caused by DNA damage from the free radicals generated during postischemic reperfusion of the blood. The aim of this study was to evaluate the cytogenetic damage caused by postischemic reperfusion on peripheral lymphocytes of five women and six men undergoing total knee arthroplasty "bloodless" operation using samples received before, during, immediately, and 1 h after the operations. The sister chromatid exchange assay was applied to peripheral blood lymphocyte cultures and the levels of sister chromatid exchanges were analyzed as a quantitative index of genotoxicity, along with the values of mitotic index and proliferation rate index as qualitative indices of cytotoxicity and cytostaticity, respectively. We observed that postischemic reperfusion induced cytogenetic damages specifically through reperfusion. DNA effects were most pronounced after tourniquet release and declined afterward without returning to preischemic baseline values. Our findings suggest the presence of a functional association between postischemic reperfusion and cytogenetic damage that may have important clinical implications.

References

Apr 11, 1992·Anesthesia and Analgesia·E C BlochD I Sessler
Jul 1, 1992·Journal of Pediatric Orthopedics·M GoodarziJ A Ogden
Aug 1, 1992·Circulation Research·G L StahlJ C Longhurst
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N C VamvakopoulosI A Kourides
Nov 1, 1994·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·N C Vamvakopoulos, T O Sioutopoulou
Jan 1, 1993·European Journal of Applied Physiology and Occupational Physiology·H J AppellJ M Soares
Jan 1, 1993·Cytogenetics and Cell Genetics·N C VamvakopoulosE W Jabs
Dec 1, 1996·British Journal of Anaesthesia·J P EstebeC Ecoffey
Oct 28, 1997·Stroke; a Journal of Cerebral Circulation·M E MackeyN T Kouchoukos
Feb 3, 2000·Free Radical Biology & Medicine·C WillyU G Plappert
Feb 15, 2001·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·P A CowledR A Fitridge
Jul 27, 2001·European Journal of Pharmacology·E MazzonS Cuzzocrea
Oct 6, 2001·American Journal of Surgery·I N Nomikos, N C Vamvakopoulos
Feb 19, 2002·The European Journal of Surgery = Acta Chirurgica·K P HatzipantelisM Albani
Feb 19, 2005·The Tohoku Journal of Experimental Medicine·Ercan CetinusNihal Buzkan
Mar 2, 2006·The Journal of Trauma·Benjamin W StarnesRobert M Rush
Apr 21, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Junko AdachiYasuhiro Ueno
Nov 23, 2006·The Journal of Surgical Research·Erhan AtahanUfuk Guney Ergun
Dec 21, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·D O SioutopoulouN C Vamvakopoulos
Apr 11, 2008·The Journal of Trauma·John F KraghJohn B Holcomb
Nov 7, 2008·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·E AthanassiouC Hatzitheofilou
Apr 18, 2009·Genetic Testing and Molecular Biomarkers·Kyriaki AkritopoulouDionysios Mourelatos
May 16, 2009·Current Diabetes Reviews·I N NomikosN C Vamvakopoulos
Jun 16, 2009·Free Radical Research·Merja Laisalmi-KokkiLeena Lindgren
Sep 11, 2009·Current Opinion in Critical Care·Chitra N Sambasivan, Martin A Schreiber
Sep 19, 2009·Pathology, Research and Practice·Maria SatraNicholas C Vamvakopoulos
Oct 13, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E DigkasTh Lialiaris
Nov 26, 2009·Genetic Testing and Molecular Biomarkers·Theodoros LialiarisVassilios Vargemezis

❮ Previous
Next ❯

Citations

Feb 4, 2012·Genetic Testing and Molecular Biomarkers·Constantinos MourelatosTheodore Lialiaris
Dec 14, 2018·Oxidative Medicine and Cellular Longevity·Prangmalee LeurcharusmeeSiriporn C Chattipakorn

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.