Cytogenetic evaluation for the genotoxicity of bisphenol-A in Chinese hamster ovary cells

Environmental Toxicology and Pharmacology
Lili XinHailin Tian

Abstract

Bisphenol A (BPA), identified as an endocrine disruptor, is an important man-made compound used in a wide range of consumer products. The MTT assay, comet assay, micronucleus test, chromosome aberration test, and Ames assay were conducted to assess the cytotoxic, genotoxic, cytogenetic effects, and mutagenic activity of BPA. After BPA exposure, we showed significant increases in cytotoxicity and level of DNA damage indicated by Olive tail moment, tail length, and % tail DNA in a similar dose- and time-dependent manner. Significant increases in micronucleus frequency and conventional chromosome aberrations were also observed after BPA treatment. The major types of structural aberrations were breaks, gaps, and fragments. However, no positive mutagenic activity of BPA was observed in any of the tester strains. Taken together, the data obtained in this study clearly demonstrated that BPA is not mutagenic but could exhibit significant genotoxic and cytogenetic effects in Chinese hamster ovary cells.

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Citations

Jul 16, 2016·Fertility and Sterility·Ayelet Ziv-Gal, Jodi A Flaws
Feb 10, 2017·Human & Experimental Toxicology·A SantovitoP Cervella
Aug 31, 2019·International Journal of Environmental Research and Public Health·Marcello DallioAlessandro Federico
Mar 30, 2021·Frontiers in Endocrinology·Giada FrenzilliNoomi Asker
Apr 23, 2021·Colloids and Surfaces. B, Biointerfaces·Mateus D MaximinoPriscila Aléssio

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