PMID: 6407472Apr 1, 1983Paper

Cytogenetic localization by variation in electrophoretic allozyme phenotype: Drosophila Odh

Biochemical Genetics
B A Clark

Abstract

A gene-dosage effect is characteristic of eukaryotic structural genes and is therefore useful in gene mapping. However, attributing quantitative variations in enzyme activity to a gene-dosage effect or other putative regulatory loci can be suspect when the locus in question may be inducible by variations in culture conditions. The problem of controlling for allele-specific variations in activity and regulation can be circumvented in Drosophila melanogaster by the use of synthetic duplications and deficiencies in conjunction with enzyme polymorphism. A method for constructing segmental aneupliods heterozygous for electrophoretic variants of octanol dehydrogenase (Odh) is presented which permitted variations in allozyme phenotype and enzyme activity--which show a strict dosage dependency--to be produced simultaneously. The structural gene region for Odh was identified using T(Y;A) stocks and the deficiency M(3)S31 was used to assign the locus to polytene band region 86D1-4. With this method a segmental aneuploid survey of Drosophila for purposes of gene localization can be accomplished in one generation with appropriate stocks.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W J Dickinson, H L Carson
Feb 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·R L SeecofD G Futch

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