Cytogenetic status of human lymphocytes after exposure to low concentrations of p,p'-DDT, and its metabolites (p,p'-DDE, and p,p'-DDD) in vitro

Chemosphere
Marko GerićVera Garaj-Vrhovac

Abstract

Despite that the use of DDT has been restricted for more than 40 years to malaria affected areas, low doses of this pesticide and its metabolites DDE and DDD can be found in the environment around the world. Although it has been shown that these pollutants induce cell and DNA damage, the mechanisms of their cytogenotoxic activity remains largely unknown. This study looks into their possible genotoxic effects, at doses that can be found in body fluids, on human lymphocytes using the cytokinesis-block micronucleus assay and the comet assay. After exposure for 1, 6, and 24 h compounds p,p'-DDT (0.1 μg mL(-1)), p,p'-DDE (4.1 μg mL(-1)), and p,p'-DDD (3.9 μg mL(-1)) showed increase in DNA damage. The most significant results were observed at exposure period of 24 h where number of micronucleated cells increased from control 2.5±0.71 to 23.5±3.54, 13.5±0.71, and 16.5±6.36 for DDT, DDE, and DDD, respectively. Similar effect was observed using comet test where the percentage of DNA in comets tail increased from control 1.81±0.16 to 17.24±0.55, 11.21±0.56 and 9.28±0.50 for each compound, respectively. At the same time Fpg-comet assay failed to report induction of oxidative DNA damage of these pollutants. Additionally, the type of cell d...Continue Reading

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Citations

Mar 10, 2015·The Science of the Total Environment·Yolanda Jasso-PinedaIvan Nelinho Pérez-Maldonado
Jun 6, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Marko GerićVera Garaj-Vrhovac
Feb 2, 2015·Ecotoxicology and Environmental Safety·Marko GerićVera Garaj-Vrhovac
Oct 26, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Marko GerićVera Garaj-Vrhovac
Aug 1, 2020·Environmental Science and Pollution Research International·Patricia Santillán-SidónCelia Vazquez-Boucard
Jan 11, 2018·Archives of Environmental Contamination and Toxicology·Davor ŽelježićJudita Grzunov Letinić

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