PMID: 2499774Jun 1, 1989Paper

Cytogenetical characterisation of Chinese hamster 43-3B transferants with the amplified or non-amplified human DNA repair gene ERCC-1

Mutation Research
F DarroudiA T Natarajan

Abstract

A comparative study on the biological responses to different mutagens (UV, 4NQO, MMC, MMS and EMS) was made on CHO wild-type cells (CHO-9), its UV-hypersensitive mutant 43-3B, and 2 types of its transferants, i.e., one containing a few copies of the human repair gene ERCC-1 and the other having more than 100 copies of ERCC-1 (due to gene amplification). Cell survival, chromosomal aberrations and SCEs were used as biological end-points. The spontaneous frequency of chromosomal aberrations in the transferants was less than found in 43-3B mutant cells, but still 2-3 times higher than in wild-type CHO cells. The spontaneous frequency of SCEs in the transferants was less than in 43-3B and similar to that of wild-type cells. The induction of SCEs by all tested agents in transferants was similar to that found in CHO-9 cells, while the mutant is known to respond with higher frequencies. ERCC-1 also bestowed resistance to MMS and EMS on the mutant to induction of chromosomal aberrations and cell killing to levels comparable with those of the wild-type strain. On the other hand ERCC-1 could not completely regain the repair proficiency against cell killing and induction of chromosomal aberrations by UV or MMC to the wild-type level. These...Continue Reading

References

Sep 1, 1976·Journal of the National Cancer Institute·J L Biedler, B A Spengler
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J H NunbergL A Chasin
Jan 1, 1985·Somatic Cell and Molecular Genetics·L H ThompsonM J Siciliano
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J D MilbrandtJ L Hamlin
Jul 1, 1984·Cell·R T Schimke
Jan 1, 1984·Annual Review of Biochemistry·G R Stark, G M Wahl
Aug 2, 1984·Nature·A WesterveldD Bootsma
Feb 1, 1980·Mutation Research·H J BurkiR D Wood

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