Cytokeratin expression in primary epithelial cell culture from bovine conjunctiva

Tissue & Cell
Grazia PaladinoVincenzo Enea

Abstract

Aim of the present study is to extend our previous observations on a model of primary epithelial cell culture obtained from bovine conjunctiva, and analyse the maintenance of the conjunctival phenotype, relative to cytokeratin (CK) expression, through extended periods of cultivation under different conditions. Conjunctival epithelial cells were grown in transwell filters, and cultured either under liquid covered (LC), or air-interface (AI) conditions. The physiological state of the cells was monitored daily by measurement of the trans-epithelial electrical resistance (TEER). Analysis of cytokeratin expression was then carried out at different time points (up until 14 days), and compared to the original profile of the conjunctival tissue in order to assess deviations from the primitive phenotype. Immunodetection studies, carried out by both western immunoblot and immunofluorescence analyses, revealed constant expression of the pan-epithelial marker AE3 (recognizing basic type cytokeratins), confirming the epithelial nature of the culture. Other cytokeratins characteristic of non-keratinized stratified epithelia (CK4 and CK13) were absent in corneal tissue, while in conjunctival epithelial cells were more expressed under AI than ...Continue Reading

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Citations

Oct 26, 2006·Annals of Biomedical Engineering·Nurit Even-TzurMichael Wolf
May 26, 2009·International Journal of Pharmaceutics·C CivialeM G Mazzone
Jan 15, 2008·Experimental Eye Research·Federico Castro-Muñozledo
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Sep 2, 2011·Journal of Cellular Physiology·Rachelle E KosoffCarlo C Maley
Mar 17, 2016·Journal of Biomaterials Applications·Xu XuQuan-Kui Lin

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