Cytokine activity after human bone marrow transplantation. III. Defect in IL2 production by peripheral blood mononuclear cells is not corrected by stimulation with Ca++ ionophore plus phorbol ester.

British Journal of Haematology
M A CooleyK Atkinson

Abstract

Previous studies have shown that interleukin 2 (IL2) production by peripheral blood mononuclear cells (PBMC) is severely impaired post allogeneic bone marrow transplantation, whereas production of interferon-gamma (IFN-gamma) is at most marginally depressed. To investigate the mechanisms behind this apparently differential inhibition of lymphokine production, we stimulated PBMC from recipients of HLA-identical sibling bone marrow transplants with phytohaemagglutinin (PHA), PHA + phorbol ester (PMA) (to bypass accessory cell requirements) or Ca++ ionophore + PMA (to bypass both accessory cell and T cell surface receptor (CD2 and/or CD3/Ti interactions). Increasing the potency of the stimulus increased the amount of IL2 and IFN produced by PBMC from both normal volunteers and from marrow transplant recipients, but for each stimulus the amount of IL2 produced by marrow transplant recipient PBMC remained 10-100-fold lower than that produced by normal PBMC, suggesting an underlying defect in IL2 production by marrow transplant recipient T cells, not due to accessory cell or CD2 defects. Selection experiments showed that CD3+ cells were the primary IL2 producers, and we were unable to demonstrate presence of suppressor cells in marro...Continue Reading

References

Jun 1, 1985·The Journal of Experimental Medicine·K A AultB R Smith
Feb 1, 1987·Australian and New Zealand Journal of Medicine·K AtkinsonG Morgan
Jan 1, 1985·Cancer Immunology, Immunotherapy : CII·P AllavenaJ R Ortaldo
Jan 21, 1972·Nature·D C ChangH E Rorschach

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Citations

Jul 16, 2003·International Immunopharmacology·James E Talmadge
Sep 1, 1996·Stem Cells·J L FerraraW Krenger
Jan 21, 2010·Journal of Medical Virology·D ChatzidimitriouE Diza
Jan 1, 1992·Growth Factors·W A SewellM A Cooley

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