Abstract
Nitric oxide causes apoptotic cell death in RAW 264.7 macrophages. The cellular response to the NO donor S-nitrosoglutathione (GSNO) comprises an apoptotic morphology and DNA fragmentation, which largely depends on the accumulation of the tumor suppressor gene product p53. Pre-treatment of macrophages with LPS, IFN-gamma in the presence of NG-monomethyl-L-arginine (NMMA) imparts resistance to apoptotic cell death, normally elicited by exogenously-supplied GSNO. Similarly, pre-treatment with low-dose GSNO (25-200 microM) conferred resistance from a second exposure to a higher dose of GSNO (1 mM). Protection is comprehended at the level of blocked p53 accumulation. Upregulation of protective mechanisms in response to non-lethal NO concentrations or by LPS, cytokine pre-stimulation may redirect the ability of nitric oxide to upregulate p53 and to initiate macrophage apoptosis, thereby modulating cellular susceptibility towards NO-intoxication.
Citations
Jun 9, 2001·Antioxidants & Redox Signaling·J D LaskinD L Laskin
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·S MohrE G Lapetina
Jul 24, 2012·Nitric Oxide : Biology and Chemistry·Lotta Renström KoskelaPetra J de Verdier
Aug 25, 2015·Redox Biology·Charlotta RykPetra J de Verdier
Nov 9, 2004·Mutation Research·Philip J CoatesEric G Wright
Jul 28, 2004·Nitric Oxide : Biology and Chemistry·Carlos del FresnoEduardo López-Collazo
May 2, 2006·Prostaglandins & Other Lipid Mediators·Isidora RanchalJordi Muntané
Mar 25, 2014·Cancer Letters·Tomas ThielPetra J de Verdier
Aug 8, 2002·Journal of Gastroenterology and Hepatology·Gu KongJong Chul Rhee
Aug 20, 2014·Archives of Biochemistry and Biophysics·Alessandro CortiAlfonso Pompella
Oct 15, 2003·Oncogene·Sally A LorimoreEric G Wright
Jun 17, 2000·Radiation Research·N V GorbunovJ S Greenberger
May 5, 2012·Annals of Thoracic Medicine·Ibrahim JanahiBalamurugan Panneerselvam
Apr 9, 2001·Transplantation·A DelikourasA Dorling
Nov 13, 2001·Oncogene·S A LorimoreE G Wright
Sep 14, 1999·The American Journal of Physiology·I PetracheA M Choi
Nov 10, 2004·Journal of Cellular Biochemistry·Kae-Yuan LinAn-Na Chiang
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B del BelloE Maellaro
Nov 24, 1999·Archives of Biochemistry and Biophysics·R S GrantV Kapoor
Apr 1, 1997·Nitric Oxide : Biology and Chemistry·K D KrönckeV Kolb-Bachofen
Aug 28, 1998·European Journal of Pharmacology·B BrüneK B Sandau
Oct 15, 1998·International Journal for Parasitology·M E SelkirkK Gounaris
Sep 26, 2013·ACS Applied Materials & Interfaces·Nicholas J SchaubRyan J Gilbert