PMID: 7547710May 1, 1995Paper

Cytokine dependence of V gamma 3 thymocytes: mature but not immature V gamma 3 cells require endogenous IL-2 and IL-7 to survive--evidence for cytokine redundancy

International Immunology
G LeclercqJ Plum

Abstract

It has previously been described that V gamma 3 cells can proliferate extensively in vitro in the presence of different cytokines. Here, the role of cytokines in the maintenance of V gamma 3 cells in the thymus has been determined. Culture of fetal thymocytes in cell suspension for 24 h showed that, whereas immature TCRlowHSAhigh V gamma 3 cells remained viable, all mature TCRhighHSAlow V gamma 3 cells died. These cells died by apoptosis since protein synthesis was required and flow cytometric analysis as well as DNA gel electrophoresis showed that the DNA was degraded to oligonucleosomal bands. Addition of IL-2, IL-4 or IL-7 to suspension cultures of fetal thymocytes rescued V gamma 3 cells from dying. Addition of IL-1, IL-3, IL-5, IL-6, IL-9, TNF-alpha or IFN-gamma was without effect. Phenotypic analysis showed that the alpha-chain of the IL-2 receptor (IL-2R alpha) was expressed by part of the immature V gamma 3 thymocytes, all mature V gamma 3 cells expressed the beta-chain of the IL-2 receptor (IL-2R beta). Addition of anti-IL-2R beta mAb to fetal thymic organ culture (FTOC) resulted in a moderate reduction of the cell number of mature V gamma 3 thymocytes. Addition of anti-IL-2R alpha, anti-IL-4 or anti-IL-7 mAb had no ef...Continue Reading

Citations

Feb 1, 1997·Current Opinion in Immunology·R Boismenu, W L Havran
Feb 13, 2007·Immunological Reviews·Rebecca L O'BrienWilli K Born
Jan 16, 2007·Developmental Biology·Eilon WoolfYoram Groner
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katrien Van BenedenGeorges Leclercq
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·An De CreusGeorges Leclercq
Apr 12, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karen LakyLynn Puddington
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T C KerreB Vandekerckhove
Jan 23, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tina Van Den BroeckGeorges Leclercq

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