Cytokine-enhanced mixed lymphocyte reaction (MLR) in cord blood

Clinical and Experimental Immunology
M UmemotoM Sakurai

Abstract

Although in cord blood (CB) transplantation graft-versus-host disease (GVHD) is reported to be less severe, GVHD may occur even in patients with HLA-identical sibling donors. This result shows that HLA typing can not entirely predict GVHD. The standard MLR with CB cells was either normal or slightly reduced compared with adult peripheral blood (PB) cells. We used two manipulations to increase the responses of CB cells to allo-antigens. The first was to treat the stimulator cells with cytokines, and the second to amplify weak proliferative responses by adding exogenous cytokines to MLR cultures (modified MLR). The stimulator cells were treated with both interferon-gamma (IFN-gamma) and IL-4. The responder cells were treated with both IL-2 and tumour necrosis factor-alpha (TNF-alpha). It is still to be determined whether or not this cytokine-enhanced MLR could be a possible predictor of GVHD. However, using these cytokines, 90% of CB could recognize allo-antigens, even if the standard MLR was negative.

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D T HarrisE A Boyse
May 1, 1991·Cellular Immunology·S R SmithD L Phillips
Mar 7, 1991·The New England Journal of Medicine·J L Ferrara, H J Deeg
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·G RisdonH E Broxmeyer
Jan 1, 1993·Annual Review of Immunology·M A Farrar, R D Schreiber
Jul 18, 1996·The New England Journal of Medicine·J KurtzbergP Rubinstein

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Citations

Jul 27, 2001·Pediatrics International : Official Journal of the Japan Pediatric Society·Q JiangY Komada
Sep 1, 2001·Clinical and Experimental Immunology·Q JiangY Komada
Feb 6, 2013·Clinical and Experimental Immunology·S IwamotoY Komada
Jul 25, 2006·Transplant Immunology·Syh-Jae LinHsiu-Shan Hsaio
Nov 17, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mariko ArakiYoshihiro Komada

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