PMID: 8955506Nov 1, 1996Paper

Cytokine expression by models of human trophoblast as assessed by a semiquantitative reverse transcription-polymerase chain reaction technique

American Journal of Reproductive Immunology : AJRI
W A BennettB D Cowan

Abstract

Cytokines form an important communication network between the mother and fetus. Defining the significance of these factors requires an understanding of their constitutive expression by maternal and fetal tissues. This study examines cytokine expression by human trophoblast. A reverse transcription polymerase chain reaction (RT-PCR) technique was used to assess cytokine expression by choriocarcinoma cells (BeWo, JEG-3, and JAR) and term trophoblast. Placental digests were enriched for trophoblast by immunoaffinity (CD-9) columns. Interleukin (IL)-1, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma were weakly expressed or absent in the choriocarcinoma cells. In contrast, these cytokines were expressed by term trophoblast. IL-6, IL-10, IFN-alpha, IFN-beta, and granulocyte macrophage-colony stimulating factor (GM-CSF) mRNA were detected in all trophoblast cells, except for a paucity of IL-10 expression by JEG-3 cells. Human choriocarcinoma cells and term trophoblast express cytokines that may regulate critical reproductive events. Expression of inflammatory cytokines such as IL-1, TNF-alpha, and IFN-gamma by term trophoblast could trigger labor or be a consequence of labor-associated events.

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Citations

Oct 7, 2000·Bulletin of Experimental Biology and Medicine·S A Sel'kovA V Selyutin
May 17, 2005·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Jacek R Wilczyński
Apr 30, 1999·Journal of Reproductive Immunology·A A de MoraesP J Hansen
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Dec 31, 1998·American Journal of Reproductive Immunology : AJRI·W A BennettB D Cowan
Dec 31, 1998·American Journal of Reproductive Immunology : AJRI·M ZygmuntU Lang
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Jun 9, 2009·Journal of Reproductive Immunology·Lorenza DíazFernando Larrea
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Margaret Saunders
Sep 10, 2010·American Journal of Reproductive Immunology : AJRI·Veronica L ScottKaren S Coats
Sep 11, 2012·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Deepika SharmaJayashree Bhattacharjee
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