Cytokine Expression of Stem Cells Originating from the Apical Complex and Coronal Pulp of Immature Teeth

Journal of Endodontics
Ki Hoon JooJae-Ho Lee

Abstract

The aim of this study was to measure and compare the expression levels of cytokines from developing apical complex cells (DACCs) and dental pulp stem cells (DPSCs) of the immature tooth. DPSC-conditioned medium (CM) and DACCs-CM were obtained from human young teeth, and 174 cytokines secreted from each CM were identified and compared. A cytokine membrane array and enzyme-linked immunosorbent assay were used to measure and compare the expression levels of the cytokines. Immunocytochemistry targeting insulin-like growth factor-1 and neurotrophin-3 was additionally performed. There were statistically significant differences in the expression levels of 25 cytokines: 22 and 3 were expressed more strongly in DPSCs-CM and DACCs-CM, respectively. Odontoblast differentiation-related cytokines were more strongly expressed in DPSCs-CM, while cell-proliferation-related cytokines were more strongly expressed in DACCs-CM. Proinflammatory and anti-inflammatory cytokines were predominantly expressed in DPSCs-CM and DACCs-CM, respectively. DPSCs may exert a stronger paracrine effect than DACCs on regeneration of the dentin-pulp complex, in terms of odontoblast differentiation.

Citations

Aug 30, 2018·Tissue Engineering. Part B, Reviews·Charlène KichenbrandVanessa Moby
Feb 9, 2020·Journal of Clinical Medicine·Dishant ShahKyounga Cheon
Feb 25, 2020·Stem Cells International·Sara El MoshyKarim M Fawzy El-Sayed
Jun 25, 2020·International Journal of Molecular Sciences·Ji Won YangHyung-Sik Kim
Nov 13, 2020·World Journal of Stem Cells·Jagadish HosmaniHarish C Chandramoorthy
Apr 9, 2021·Stem Cell Reviews and Reports·Vincenzo MatteiSimona Delle Monache
Nov 1, 2020·Archives of Oral Biology·Sijing YuBo Gao
May 18, 2021·Journal of Oral Biology and Craniofacial Research·Damrong DamrongsriThanaphum Osathanon

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