Cytokine expression profiles of bovine lymph nodes: effects of Mycobacterium bovis infection and bacille Calmette-Guérin vaccination.

Clinical and Experimental Immunology
Stephanie WiddisonT J Coffey

Abstract

Cytokine expression in lymph nodes from cattle inoculated intranasally with Mycobacterium bovis was compared to that of non-infected animals using real-time polymerase chain reaction. The effect of M. bovis infection, 4 months post-challenge, was to suppress the expression of anti-inflammatory cytokines interleukin (IL)-4 and IL-10 as well as the pro-inflammatory cytokines tumour necrosis factor (TNF) and IL-6. Expression of interferon (IFN)-gamma and IL-12 was maintained. Animals vaccinated with bacille Calmette-Guérin responded differently to challenge with M. bovis. In particular, no decrease in expression of IL-4 or IL-6 was observed following challenge of vaccinated animals and decreased IFN-gamma was detected. Also, vaccinated animals had higher levels of IL-4 and IL-10 transcripts compared to unvaccinated animals following challenge. These changes in cytokine expression levels led to a significant shift in the IFN-gamma/IL-4 or IFN-gamma/IL-10 ratio within the lymph node following challenge. Challenged animals generally showed a strong Th1 bias that was not seen in animals vaccinated prior to challenge. An inverse correlation between the level of pathology and bacterial load within the lymph node and the expression of IL...Continue Reading

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Citations

Jul 21, 2010·BMC Genomics·Stephanie WiddisonTracey J Coffey
May 27, 2014·Veterinary Immunology and Immunopathology·Federico Carlos BlancoMarcelo Abel Soria
Jan 15, 2011·Developmental and Comparative Immunology·Stephanie WiddisonTracey J Coffey
Aug 11, 2010·Veterinary Immunology and Immunopathology·J WitchellM Goyal
Mar 6, 2007·Veterinary Immunology and Immunopathology·Stephanie WiddisonTracey J Coffey
Jun 3, 2009·Transboundary and Emerging Diseases·D E MacHughK G Meade
Nov 26, 2008·Veterinary Immunology and Immunopathology·Janice J EndsleyD Mark Estes
Aug 5, 2008·Tuberculosis·Hima Chandana Boddu-JasmineMadhu Goyal
Dec 28, 2010·Tuberculosis·Federico Carlos BlancoFabiana Bigi
Nov 6, 2007·Pathology, Research and Practice·Jaydene WitchellMadhu Goyal
Aug 8, 2009·Microbiology and Immunology·Federico C BlancoFabiana Bigi
Sep 19, 2019·Infection and Immunity·Kathryn WrightKumudika de Silva
Jul 4, 2017·Veterinary Immunology and Immunopathology·Michael P SheridanEamonn Gormley

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