PMID: 9652729Jul 4, 1998Paper

Cytokine gene activity in AML cells in vivo in patients

Leukemia Research
X Z GaoH D Preisler

Abstract

The proliferation of acute myelogenous leukemia cells is dependent upon cytokine stimulation. Additionally, there is a body of literature which reports that leukemia cells produce GMCSF, IL6, and other cytokines. The study reported here, using an rt-multiplex polymerase method, determined the presence or absence of transcripts in freshly obtained AML cells for the following cytokine or cytokine-related genes: IL 1beta, IL1ra, TNF alpha, GMCSF, IL6, flt 3, and hSCF. This demonstrated that leukemia cell populations usually contain transcripts for IL1beta, TNF alpha, flt 3 and flt 3 ligand in vivo and that transcripts for the other cytokines only appear after the leukemia cells are processed in vitro. The presence of TNF alpha transcripts appears to be associated with resistance to remission induction therapy. Furthermore, the transcript profile of the leukemia cells can change during remission induction therapy. The data also demonstrate the assessment of cytokine production by leukemia cells after in vitro manipulation should not be extrapolated to the in vivo situation.

References

Sep 1, 1992·Blood Reviews·N H Russell
Feb 1, 1994·PCR Methods and Applications·M C Edwards, R A Gibbs

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Citations

Nov 26, 1999·Cancer·H D Preisler
Sep 30, 2006·Blood·Wenlin YuanTimothy J Ley
Mar 8, 2000·Leukemia & Lymphoma·H D PreislerA Raza
Oct 28, 2003·The American Journal of Pathology·Sofie StruyfGeneviève Laureys
Jul 13, 2002·Expert Review of Anticancer Therapy·Apostolia-Maria Tsimberidou, Francis J Giles
Jun 3, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·George F Vande WoudeWilliam J Slichenmyer

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