Cytokine-induced killer cells against autologous CLL: direct cytotoxic effects and induction of immune accessory molecules by interferon-gamma

International Journal of Cancer. Journal International Du Cancer
Martin KornackerAnthony D Ho

Abstract

Cytokine-induced killer cells (CIK cells), coexpressing CD3 and CD56, can be expanded from peripheral blood mononuclear cells by the timed addition of interferon-gamma (IFN-gamma), IL-2 and OKT3. The effects of CIK cells on primary, autologous CLL cells are described. We used MACS to separate CD3(+) cells for expansion of CIK cell effectors and CD19(+) targets from peripheral blood of 16 CLL patients. Apoptosis was assessed by measuring annexinV staining in CLL cells. After incubation of autologous CIK with CLL, specific apoptosis in CLL cells was 15%. Coincubation with irradiated CIK cells for 48 hr before adding vital CIK cells resulted in an increased ICAM-1 expression on CLL cells and an increase in apoptosis of CLL targets (30%). These effects were mediated by IFN-gamma secretion of CIK cells. In addition to their direct cytotoxic effect, CIK cells secrete IFN-gamma that modulates the expression of adhesion molecules on CLL cells, and this enhances apoptosis induction by cytotoxic effector cells.

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Citations

Dec 20, 2007·Archives of Pharmacal Research·Hwan Mook KimSang-Bae Han
May 10, 2007·Cancer Immunology, Immunotherapy : CII·Freddy Tita-NwaMartin Kornacker
May 28, 2009·Archives of Pharmacal Research·Hwan Mook KimSang-Bae Han
Dec 1, 2010·Archives of Pharmacal Research·Yeon Jin KimSang-Bae Han
Aug 13, 2010·Cancer Biotherapy & Radiopharmaceuticals·Xiaosan SuTao Guo
Apr 30, 2015·Immune Network·Ji Sung KimSang-Bae Han
Dec 25, 2008·International Immunopharmacology·Hwan Mook KimSang-Bae Han
Apr 2, 2011·Clinical & Developmental Immunology·Yao WangWeidong Han
Nov 13, 2007·International Immunopharmacology·Hwan Mook KimSang-Bae Han
Nov 13, 2007·International Immunopharmacology·Hwan Mook KimSang-Bae Han
Jan 27, 2015·Oncology Letters·Xin-Yu YangFang-Ping Chen
Apr 7, 2010·Journal of Biomedicine & Biotechnology·Yeh Ching Linn, Kam M Hui
May 16, 2007·Bone Marrow Transplantation·A A Kennedy-Nasser, C M Bollard
Jan 26, 2018·International Journal of Molecular Sciences·Martino Introna, Fabio Correnti

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