Cytokine-induced killer cells co-cultured with dendritic cells loaded with the protein lysate produced by radiofrequency ablation induce a specific antitumor response

Oncology Letters
Chan-Chan ShanChang-Ping Wu

Abstract

Radiofrequency ablation (RFA) causes coagulative necrosis of tumor tissue and the production of local tumor protein debris. These fragments of tumor protein debris contain a large number of various antigens, which can stimulate a specific cellular immune response. In the present study, dendritic cells (DCs) were loaded with tumor protein lysate antigens that were produced in situ by RFA, and were used to treat murine colon carcinoma in combination with cytokine-induced killer (CIK) cells. Subsequent to the treatment of murine colon carcinoma by RFA, the in situ supernatant of tumor lysis was collected and the DCs were loaded with the lysate antigen to generate Ag-DCs. CIK cells induced from the spleen cells of mice were co-cultured with Ag-DCs to generate Ag-DC-CIK cells. The results revealed that the Ag-DC-CIK cells exhibited strong antitumor activity in vitro and in vivo. The morphology and immunophenotypes of these cells were determined using microscopy and flow cytometry, respectively. The cytotoxic activity of Ag-DC-CIK cells was determined using a CCK-8 assay. To establish a mouse model, mice were randomized into Ag-DC-CIK, DC-CIK, CIK and PBS control groups and monitored for tumor growth and survival time. ANOVA was used...Continue Reading

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Citations

Dec 2, 2017·The Journal of Experimental Medicine·Megan L SulcinerDipak Panigrahy
Jun 30, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jaimie ChangDipak Panigrahy
Jul 22, 2017·Frontiers in Immunology·Xingchun GaoWeilin Jin
Jun 2, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Amir TajbakhshAmirhossein Sahebkar

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Methods Mentioned

BETA
surgical resection
fluorescence activated cell sorting
protein assay
flow cytometry
FACS

Software Mentioned

FlowJo
GraphPad Prism

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