Cytokine-induced protein tyrosine phosphorylation is essential for cytokine priming of human eosinophils

The Journal of Allergy and Clinical Immunology
T van der BruggenL Koenderman

Abstract

Human eosinophils are strongly modulated by the eosinophilotrophic cytokines IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor (GM-CSF). A clear intracellular effect of these cytokines is the induction of tyrosine phosphorylation of multiple cellular substrates. However, the relevance of tyrosine phosphorylation for eosinophil functioning has not been established. In this study we have investigated dose-response and time curves of IL-5-, IL-3-, and GM-CSF-induced tyrosine phosphorylation in eosinophils. Moreover, we have evaluated the importance of IL-5-induced tyrosine phosphorylation for priming of human eosinophils. Cytokine-induced tyrosine phosphorylation was monitored on western blot with an antiphosphotyrosine antibody (4G10). To probe the relevance of tyrosine phosphorylation for priming, eosinophils were primed with IL-5 in the presence of the tyrosine kinase inhibitor herbimycin A. Platelet activating factor (PAF) was used as a control priming agent. Subsequently, the eosinophils were incubated with serum-treated zymosan (STZ) to activate the respiratory burst. Binding of STZ was determined by FACS analysis. IL-5-, IL-3-, and GM-CSF-induced tyrosine phosphorylation was found at concentrations that prime...Continue Reading

References

Nov 1, 1975·The Journal of Clinical Investigation·I M GoldsteinG Weissmann
Dec 1, 1992·American Journal of Respiratory Cell and Molecular Biology·R A WarringaL Koendermann
Aug 1, 1990·British Journal of Haematology·H EnokiharaK Takatsu
Feb 1, 1990·The Journal of Allergy and Clinical Immunology·G J Gleich
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J Gomez-CambroneroR I Sha'afi
Aug 15, 1989·Journal of Immunological Methods·T T HanselR A Thompson
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A F LopezM A Vadas
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·S YousefiH U Simon
Mar 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Kapp
Jan 27, 1995·Journal of Immunological Methods·M BlomA J Verhoeven
Apr 1, 1994·The European Respiratory Journal·C KroegelH Matthys
Mar 1, 1994·The European Respiratory Journal·C KroegelJ A Warner
Feb 10, 1994·Journal of Immunological Methods·M IdeG J Gleich
Aug 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T van der Bruggen, L Koenderman

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Citations

May 5, 1999·Current Opinion in Hematology·G J Roboz, S Rafii
Oct 27, 1998·Allergy·A P Kaplan, P Kuna
Jun 12, 2013·European Journal of Pharmacology·Javier MilaraJulio Cortijo
Apr 29, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Konrad PazdrakAlexander Kurosky
May 23, 2001·Journal of Agricultural and Food Chemistry·K H RouxS K Sathe
Apr 20, 2001·Journal of Agricultural and Food Chemistry·S K SatheK H Roux

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