Cytokine induction of monocyte chemoattractant protein-1 gene expression in human endothelial cells depends on the cooperative action of NF-kappa B and AP-1

European Journal of Immunology
T MartinR R Cobb

Abstract

Chemokines are potent mediators of cell migration and activation and therefore play an essential role in early events of inflammation. In conjunction with cell adhesion molecules, chemokines help to localize cells to a specific site and enhance the inflammatory reaction at the site. Clinically, elevated levels of chemokines have been found in a variety of inflammatory diseases. The prototype C-C chemokine is monocyte chemoattractant protein-1 (MCP-1) which is synthesized by variety of cell types including endothelial cells in response to a variety of stimuli. MCP-1 is a major chemoattractant for monocytes, T lymphocytes, and basophils. In the present study, we investigated the factors involved in cytokine-induced MCP-1 gene expression in human endothelial cells. We present evidence that the nuclear factor (NF)-kappa B-like binding site and the AP-1 binding site located 90 and 68 base pairs upstream of the transcriptional start site, respectively, are required for maximal induction of the human MCP-1 promoter by interleukin-(IL)-1 beta. Site-directed mutagenesis or deletion of the NF-kappa B-like site decreased the cytokine-induced activity of the promoter. Site-directed mutagenesis of the AP-1 binding site also decreased the cy...Continue Reading

References

May 1, 1991·Cytokine·T J Schall
Jan 1, 1991·Annual Review of Immunology·J J OppenheimK Matsushima
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S Ylä-HerttualaD Steinberg
Mar 1, 1990·Immunology Today·E J Leonard, T Yoshimura
Jun 15, 1990·Biochemical and Biophysical Research Communications·Y J ShyyP E Kolattukudy
Nov 1, 1989·Molecular and Cellular Biology·B J RollinsG G Wong
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Feb 1, 1994·The Journal of Experimental Medicine·M A ReadT Collins
Sep 1, 1994·The Journal of Experimental Medicine·E MartinQ W Xie
May 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·G C Parry, N Mackman
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·M W CarrT A Springer
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T MatsusakaS Akira
Apr 30, 1993·Biochemical and Biophysical Research Communications·Y J ShyyP E Kolattukudy

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Citations

Oct 12, 2000·Biochemical and Biophysical Research Communications·K MuraoJ Takahara
Jun 21, 2005·Cellular and Molecular Neurobiology·Michal ToborekAvindra Nath
Apr 16, 2013·The International Journal of Biochemistry & Cell Biology·Xiaoling DengLinhua Pang
May 16, 2002·Fertility and Sterility·Anne Van LangendoncktJacques Donnez
May 29, 2002·The Journal of Nutritional Biochemistry·Yong Woo LeeMichal Toborek
Jun 24, 1999·Cytokine & Growth Factor Reviews·E Van CoillieG Opdenakker
Oct 4, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mary Hummel, Michael M Abecassis
Dec 6, 2012·Journal of Agricultural and Food Chemistry·Chia-Chi ChuangMichael K McIntosh
Mar 25, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·G KrishnaswamyD S Chi
Sep 27, 2013·Journal of Medicinal Food·Kuan-Hung LinPi-Yu Chao
Jan 1, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ho Jun ChinDong-Wan Chae
May 24, 2005·American Journal of Physiology. Heart and Circulatory Physiology·John P CullenEileen M Redmond
Nov 9, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ahmed A ElmarakbyDavid M Pollock
Mar 4, 2011·American Journal of Physiology. Renal Physiology·Montserrat M Diaz EncarnacionJoseph P Grande
Nov 24, 2011·Experimental Diabetes Research·Mi-Young KimYong Ho Ahn
Mar 20, 2010·American Journal of Respiratory Cell and Molecular Biology·Ravisankar A RamadasAnn Marie LeVine
Oct 19, 2010·Journal of Leukocyte Biology·Christine HappelThomas J Rogers
Jul 17, 2012·Journal of Cell Science·Akiko MammotoDonald E Ingber
Sep 17, 2008·Environmental Health Perspectives·Naomi K FukagawaBrooke T Mossman

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