PMID: 8611466Apr 1, 1996Paper

Cytokine levels in platelet concentrates: quantitation by bioassays and immunoassays

British Journal of Haematology
M WadhwaR Thorpe

Abstract

Some adverse reactions to the transfusion of platelet concentrates (PCs) cannot be attributed to antibodies against blood cells or to subclinical microbial agents. It has been suggested that leucocyte-derived inflammatory cytokines such as interleukin (IL)-1, IL-6 and tumour necrosis factor (TNF) may contribute to a larger number of unexplained non-antibody-mediated adverse reactions. Three types of PCs, containing different levels of leucocytes, are currently produced. Filtration is used on demand to further reduce leucocyte contamination of these components. we have monitored the plasma of PCs prepared by the platelet-rich plasma method (PRP), the buffy-coat method or by apheresis for IL-6, IL-1, transforming growth factor-beta (TGF-beta), TNF and interferon gamma (IFN gamma). Biologically active IL-6 increased in stored PRP-PCs from a mean of 140 pg/ml on day 1 to 2395 pg/ml on day 5/6. Elevated levels of IL-8, as detected by immunoassay, were evident in PRP-PCs during routine storage under blood bank conditions. Small amounts of immunoreactive IL-1 with only minimal biological activity were present in some PRP-PCs by day 5/6. No significant increase in the levels of IL-8, IL-6 or IL-1 were seen in buffy-coat PCs during stor...Continue Reading

Citations

Jun 9, 2004·Blood Reviews·Joanna Mary Heal, Neil Blumberg
Feb 6, 1997·Transfusion Science·J Seghatchian, P Krailadsiri
Nov 25, 1998·British Journal of Haematology·L EdvardsenH J Nielsen
Oct 24, 1998·Vox Sanguinis·D GreyJ A Lown
Feb 24, 2000·Transfusion·F FrabettiR Conte
Jun 6, 2009·Transfusion·Maitane Pérez-IlzarbeJosé Antonio Pérez-Simón
Dec 14, 2011·Journal of Tissue Engineering and Regenerative Medicine·Antje MietschAnita Ignatius

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